Praskova Eva, Voslarova Eva, Siroka Zuzana, Macova Stanislava, Plhalova Lucie, Bedanova Iveta, Marsalek Petr, Pistekova Vladimira, Svobodova Zdenka
Department of Veterinary Public Health and Toxicology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic.
Neuro Endocrinol Lett. 2011;32 Suppl 1:117-20.
Ketoprofen is a common human medicine from a class of non-steroidal anti-inflammatory drugs (NSAIDs), which is provably detected in surface waters in concentrations ordinarily in μg.L-1. The aim of this study was to compare the acute toxicity of ketoprofen to embryonic and juvenile stages of aquarium fish - zebrafish (Danio rerio).
Tests were performed according to the methods of the Organisation for Economic Co-operation and Development (OECD) No. 203 (Fish, acute toxicity test) and OECD No. 212 (Fish, short-term toxicity test on embryo and sac-fry stages).
The results showed (mean ± SD) LC50 value of ketoprofen to be 632.30 ± 10.10 mg.L-1 in juvenile zebrafish and 6.44 ± 2.22 mg.L-1 in embryonic stages of zebrafish. The results revealed statistically significantly higher sensitivity (p<0.01) of the embryonic stages of zebrafish to ketoprofen compared to its juveniles. The susceptibility of embryos depends on many factors, especially yet improperly developed enzymatic system in embryos, different ways of the absorption of the substance into the organism or differences in metabolism pathways.
The acute toxicity of ketoprofen for juvenile stages of zebrafish is low, but the substance seems to be toxic for embryonic stages.
酮洛芬是一种常见的非甾体抗炎类人用药物，在地表水中能够被检测到，其浓度通常为μg.L-1。本研究的目的是比较酮洛芬对水族馆鱼类——斑马鱼（Danio rerio）胚胎期和幼鱼期的急性毒性。
测试按照经济合作与发展组织（OECD）第203号方法（鱼类，急性毒性试验）和OECD第212号方法（鱼类，胚胎和仔鱼期短期毒性试验）进行。
结果显示（平均值±标准差），酮洛芬对斑马鱼幼鱼的半数致死浓度（LC50）值为632.30±10.10mg.L-1，对斑马鱼胚胎期的LC50值为6.44±2.22mg.L-1。结果表明，与幼鱼相比，斑马鱼胚胎期对酮洛芬的敏感性在统计学上显著更高（p<0.01）。胚胎的易感性取决于许多因素，尤其是胚胎中酶系统发育不完善、物质进入生物体的吸收方式不同或代谢途径存在差异。
酮洛芬对斑马鱼幼鱼期的急性毒性较低，但该物质对胚胎期似乎具有毒性。
