寡核苷酸d(ATGCGCAT)2的晶体结构与溶液结构：X射线与核磁共振联合研究
Crystal and solution structures of the oligonucleotide d(ATGCGCAT)2: a combined X-ray and NMR study.
作者信息
Clark G R, Brown D G, Sanderson M R, Chwalinski T, Neidle S, Veal J M, Jones R L, Wilson W D, Zon G, Garman E
机构信息
Cancer Research Campaign Biomolecular Structure Unit, Institute of Cancer Research, Sutton, Surrey, UK.
出版信息
Nucleic Acids Res. 1990 Sep 25;18(18):5521-8. doi: 10.1093/nar/18.18.5521.
Abstract
A combined crystal-structure determination and NMR analysis of the octanucleotide d(ATGCGCAT)2 is reported. The X-ray analysis shows that the structure is A-form duplex in crystal state. The NMR study shows that in solution this sequence is B-type. The conformational results from each technique are presented in detail. The implications of these findings in terms of conformational flexibility and ligand binding are discussed.
摘要
报道了八聚体d(ATGCGCAT)2的晶体结构测定与核磁共振分析相结合的研究。X射线分析表明，该结构在晶体状态下为A-型双链体。核磁共振研究表明，在溶液中该序列为B-型。详细介绍了每种技术的构象结果。讨论了这些发现对于构象灵活性和配体结合的意义。
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