Tanaka Minoru, Miyajima Atsushi
Laboratory of Cell Growth and Differentiation, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo, Japan.
Methods Mol Biol. 2012;826:25-32. doi: 10.1007/978-1-61779-468-1_3.
Hepatoblasts are considered to be liver stem/progenitor cells in the fetus because they propagate and differentiate into two types of liver epithelial cells, hepatocytes and cholangiocytes. In adults, oval cells that emerge in severely injured liver are considered facultative hepatic stem/progenitor cells. However, the nature of oval cells has remained unclear for long time due to the lack of a method to isolate them. It has also been unclear whether liver stem/progenitor cells exist in normal adult liver. Recently, we and others have successfully identified oval cells and adult liver stem/progenitor cells. Here, we describe the identification and isolation of mouse liver stem/progenitor cells by utilizing antibodies against specific cell surface marker molecules.
肝母细胞在胎儿期被认为是肝脏干细胞/祖细胞，因为它们能够增殖并分化为两种类型的肝脏上皮细胞，即肝细胞和胆管细胞。在成体中，严重损伤的肝脏中出现的卵圆细胞被认为是兼性肝干细胞/祖细胞。然而，由于缺乏分离卵圆细胞的方法，其性质长期以来一直不清楚。正常成体肝脏中是否存在肝干细胞/祖细胞也不明确。最近，我们和其他研究人员成功鉴定出了卵圆细胞和成年肝脏干细胞/祖细胞。在此，我们描述了利用针对特定细胞表面标记分子的抗体对小鼠肝脏干细胞/祖细胞进行鉴定和分离的方法。
