Al-Ghamdi Thamer Hadi, Atta Ihab Shafek, El-Refaei Mohamed
College of Medicine, Al Baha University, Alaqiq, Saudi Arabia.
Faculty of Medicine, Al-Azhar University, Assuit, Egypt.
Clin Exp Hepatol. 2020 Feb;6(1):42-48. doi: 10.5114/ceh.2020.93055. Epub 2020 Feb 17.
Liver regeneration after hemi-hepatectomy may be affected by several growth factors and cytokines. The aim is to evaluate the importance of interleukin 6 (IL-6) in the induction of liver cell regeneration and find correlations with other parameters such as liver enzymes, and DNA analysis by flow cytometric studies.
80 adult male Sprague-Dawley rats were obtained and divided into two equal groups ( = 40 rats) to undergo 70% partial hepatectomy: group 1 - untreated (control) group; 40 rats not treated; and group 2 - treated group, 40 rats treated with IL-6 35 μg/100 gm body weight according to a lethality study for a period of 4 days, then hepatic resection was carried out according to the steps of Higgins and Anderson. Assessment of liver enzymes and bilirubin level was done. Flow cytometric study was done using a flow cytometer (FACSCalibur; Becton Dickinson) and DNA content was estimated with CellQuest software (Becton Dickinson).
The levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were significantly higher in the untreated group of rats with liver resection. A higher value of bilirubin was observed in the treated group. Rat weight at sacrification was significantly lower in the group of rats treated with IL-6 than those without treatment, < 0.001. Liver weight at sacrification was significantly higher in the group of rats treated with IL-6 ( < 0.001). The percentage of apoptotic cells with hypodiploid DNA content was determined from DNA histograms. Untreated rat resected liver showed a peak pattern that represented liver damage with high damage of 73.4%.
Interleukin 6 is of value in induction of liver cell regeneration after seventy percent hemi-hepatectomy as evident by increased liver cell mass, liver enzymes and flow cytometric analysis.
半肝切除术后的肝脏再生可能受到多种生长因子和细胞因子的影响。本研究旨在评估白细胞介素6（IL-6）在诱导肝细胞再生中的重要性，并寻找其与其他参数（如肝酶）的相关性，以及通过流式细胞术研究进行DNA分析。
获取80只成年雄性Sprague-Dawley大鼠，将其分为两组（每组 = 40只大鼠）进行70%部分肝切除术：第1组为未处理（对照）组，40只大鼠未接受处理；第2组为处理组，40只大鼠根据致死性研究，以35 μg/100 gm体重的剂量接受IL-6治疗，持续4天，然后按照Higgins和Anderson的步骤进行肝切除。对肝酶和胆红素水平进行评估。使用流式细胞仪（FACSCalibur；Becton Dickinson）进行流式细胞术研究，并使用CellQuest软件（Becton Dickinson）估计DNA含量。
肝切除未处理组大鼠的丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）和碱性磷酸酶（ALP）水平显著更高。处理组观察到更高的胆红素值。接受IL-6治疗的大鼠组在处死后的体重显著低于未治疗组，P < 0.001。接受IL-6治疗的大鼠组在处死后的肝脏重量显著更高（P < 0.001）。根据DNA直方图确定具有亚二倍体DNA含量的凋亡细胞百分比。未处理的大鼠切除肝脏显示出一个峰值模式，代表肝脏损伤，损伤率高达73.4%。
白细胞介素6在70%半肝切除术后诱导肝细胞再生中具有重要价值，这在肝细胞质量增加、肝酶变化和流式细胞术分析中得到了明显体现。
