Banas Agnieszka
Laboratory of Molecular Biology, Institute of Obstetrics and Medical Rescue, University of Rzeszów, Faculty of Medicine, Rzeszow, Poland.
Methods Mol Biol. 2012;826:61-72. doi: 10.1007/978-1-61779-468-1_6.
There is a great interest in the development of functional hepatocytes in vitro from different types of stem cells. Multipotential mesenchymal stem cells (MSC) compose a great source for stem cell based therapy, especially, because they can be obtain from patients own tissues, sidestepping immunocompatibility and ethical issues. Among MSCs from different sources, adipose-tissue-derived mesenchymal stem cells (AT-MSCs) are very promising because of their high accessibility, proliferation ability, potentiality, and immunocompatibility.AT-MSCs can be easily isolated from stroma vascular fraction (SVF) of adipose tissue. They represent a heterogeneous population of cells. The precise AT-MSCs's marker profile has not been defined yet; therefore, it is still not obvious how to purify these heterogeneous fraction of cells. We postulate that one of the markers defining MSC provenance is CD105 (endoglin).Therefore, we have sorted CD105(+) fraction of AT-MSCs, expanded them, and differentiated toward hepatic-like cells. In order to check their potentiality, we have firstly differentiated sorted CD105(+) AT-MSCs toward mesoderm lineages, using commercialized protocols.We have shown here, that pure CD105(+) AT-MSCs fraction revealed higher homogeneity and differentiation potential toward adipogenic, osteogenic, and chondrogenic lineages and highly inducible into the hepatogenic lineage.Generated (by using our hepatic differentiation protocol) CD105(+) AT-MSCs-derived hepatic-like cells expressed hepatocyte markers, enzymes, and functions.
人们对从不同类型干细胞体外培养功能性肝细胞有着浓厚兴趣。多能间充质干细胞（MSC）构成了基于干细胞治疗的重要来源，特别是因为它们可以从患者自身组织中获取，从而避开免疫相容性和伦理问题。在不同来源的MSC中，脂肪组织来源的间充质干细胞（AT-MSC）因其易于获取、增殖能力、潜能和免疫相容性而非常有前景。AT-MSC可以很容易地从脂肪组织的基质血管成分（SVF）中分离出来。它们代表了一群异质性细胞。精确的AT-MSC标志物谱尚未确定；因此，如何纯化这些异质性细胞部分仍不明确。我们推测定义MSC来源的标志物之一是CD105（内皮糖蛋白）。因此，我们对AT-MSC的CD105(+)部分进行了分选、扩增，并使其向肝样细胞分化。为了检测它们的潜能，我们首先使用商业化方案将分选的CD105(+) AT-MSC向中胚层谱系分化。我们在此表明，纯CD105(+) AT-MSC部分显示出更高的同质性以及向脂肪生成、成骨和成软骨谱系的分化潜能，并且高度可诱导分化为肝生成谱系。（通过使用我们的肝分化方案）生成的CD105(+) AT-MSC来源的肝样细胞表达肝细胞标志物、酶和功能。
