从间充质细胞群体中分离成人多能干细胞及其在肝脏损伤中的应用。

Isolation of adult human pluripotent stem cells from mesenchymal cell populations and their application to liver damages.

作者信息

Wakao Shohei, Kitada Masaaki, Kuroda Yasumasa, Dezawa Mari

机构信息

Department of Stem Cell Biology and Histology, Tohoku University Graduate School of Medicine, Sendai, Japan.

出版信息

Methods Mol Biol. 2012;826:89-102. doi: 10.1007/978-1-61779-468-1_8.

DOI:10.1007/978-1-61779-468-1_8

PMID:22167642

Abstract

We have found a novel type of pluripotent stem cells, Multilineage-differentiating stress enduring (Muse) cells that can be isolated from mesenchymal cell populations. Muse cells are characterized by stress tolerance, expression of pluripotency markers, self-renewal, and the ability to differentiate into endodermal-, mesodermal-, and ectodermal-lineage cells from a single cell, demonstrating that they are pluripotent stem cells. They can be isolated as cells positive for stage-specific embryonic antigen-3, a human pluripotent stem cell marker. Here, we introduce the isolation method for Muse cells and the effect of transplantation of these cells on chronic liver diseases.

摘要

我们发现了一种新型的多能干细胞，即多谱系分化应激耐受（Muse）细胞，它可从间充质细胞群体中分离出来。Muse细胞的特征包括应激耐受性、多能性标志物的表达、自我更新能力以及从单个细胞分化为内胚层、中胚层和外胚层谱系细胞的能力，这表明它们是多能干细胞。它们可以作为阶段特异性胚胎抗原-3（一种人类多能干细胞标志物）阳性的细胞被分离出来。在此，我们介绍Muse细胞的分离方法以及这些细胞移植对慢性肝病的影响。