Fundación Oftalmológica del Mediterráneo, Bifurcación Pio Baroja-General Avilés, s/n 46015, Valencia, Spain.
Graefes Arch Clin Exp Ophthalmol. 2010 Jul;248(7):985-90. doi: 10.1007/s00417-009-1289-x. Epub 2010 Mar 6.
The pathophysiology of the early events leading to diabetic retinopathy is not fully understood. It has been suggested that Inflammatory processes are involved in the development of the disease; however, the concentrations of tissue retinal inflammatory mediators and their possible alteration in diabetic retinopathy have not been described. The aim of this work was to study T-helper cell cytokine and chemokine profiles, and tyrosine nitration in retinal tissue of diabetic rats.
Cytokines (interleukin IL-1a, IL-1b, IL-2, IL-4, IL-6, IL-10, TNFa, GM-CSF, IFN-g), chemokines (MIP-1a, MIP-2, MIP-3a, MCP-1, GRO/KC, RANTES, Fractalkine), and tyrosine nitration were measured in retinal homogenate obtained from Long-Evans rats after 5 months of experimental diabetes.
The T-helper type 1 cytokines IL-2 and INF-gamma, in addition to NO production (measured as nitrotyrosine), were found to be significantly elevated in diabetic rat retina homogenates. None of the other cytokines and chemokines studied were affected by the diabetic condition.
Immunoregulatory cytokines belonging to the Th-1 group (IL-2 and IFN-gamma) were increased in the retina of experimental diabetic rats. Moreover, the nitrotyrosine formation (as an expression of increased NO production) was significantly elevated in the diabetic retina, supporting the concept of an inflammatory element in the development of diabetic retinopathy.
导致糖尿病性视网膜病变的早期事件的病理生理学尚未完全了解。已经有人提出炎症过程与疾病的发展有关；然而，尚未描述组织视网膜炎症介质的浓度及其在糖尿病性视网膜病变中的可能变化。这项工作的目的是研究糖尿病大鼠视网膜组织中 T 辅助细胞细胞因子和趋化因子的特征以及酪氨酸硝化。
在实验性糖尿病 5 个月后，从 Long-Evans 大鼠的视网膜匀浆中测量细胞因子（白细胞介素 IL-1a、IL-1b、IL-2、IL-4、IL-6、IL-10、TNFa、GM-CSF、IFN-g）、趋化因子（MIP-1a、MIP-2、MIP-3a、MCP-1、GRO/KC、RANTES、Fractalkine）和酪氨酸硝化。
发现 T 辅助型 1 细胞因子 IL-2 和 INF-gamma 以及 NO 产生（如硝基酪氨酸所示）在糖尿病大鼠视网膜匀浆中显着升高。研究的其他细胞因子和趋化因子均不受糖尿病状态的影响。
属于 Th-1 组的免疫调节细胞因子（IL-2 和 IFN-gamma）在实验性糖尿病大鼠的视网膜中增加。此外，糖尿病视网膜中硝基酪氨酸的形成（作为 NO 产生增加的表达）显着升高，支持了糖尿病性视网膜病变发展中炎症因素的概念。
