Zhi Ya-Qin, Zhang Yi-Zhuo, Zhao Hai-Feng, Zhao Dan-Dan, Xia Bing, Wu Xiao-Xiong, Da Wan-Ming
Department of Hematology, Tianjin Medical University Cancer Hospital, Tianjin, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2011 Dec;19(6):1438-42.
The study was aimed to investigate the expression of stromal cell derived factor (SDF-1) in bone marrow (BM) and its relation with apoptosis of BM CD34(+) cell and angiogenesis in myelodysplastic syndrome (MDS). 40 patients with MDS were divided into low-risk group and high-risk group according to IPSS score system. BM samples were collected. SDF-1 levels, the apoptosis of CD34(+) cells and microvessel density (MVD) of BM were detected by ELISA, flow cytometry and immunochemistry, respectively. The results showed that the SDF-1 level in MDS patients was significantly higher than that in normal controls(p < 0.05), and SDF-1 level in low-risk group was significantly higher than that in high-risk group. Apoptosis of CD34(+) cells significantly increased in low-risk group compared with other groups (p < 0.05). MVD in BM biopsy significantly increased in high-risk MDS group (p < 0.05), compared with low-risk MDS group which also had higher MVD than the control group (p < 0.05). Positive correlation was found between apoptosis of CD34(+) cells and SDF-1 levels in low-risk group, and SDF-1 level and MVD in high-risk group. It is concluded that the expression of SDF-1, apoptosis of BM CD34(+) cells and MVD were significantly abnormal in MDS patients, especially in different risk group, suggesting that SDF-1 level is related to cell apoptosis and angiogenesis.
本研究旨在探讨基质细胞衍生因子（SDF-1）在骨髓增生异常综合征（MDS）患者骨髓中的表达及其与骨髓CD34(+)细胞凋亡和血管生成的关系。40例MDS患者根据国际预后评分系统（IPSS）分为低危组和高危组。采集骨髓样本，分别采用酶联免疫吸附测定（ELISA）、流式细胞术和免疫化学方法检测SDF-1水平、CD34(+)细胞凋亡率和骨髓微血管密度（MVD）。结果显示，MDS患者骨髓中SDF-1水平显著高于正常对照组（p < 0.05），且低危组SDF-1水平显著高于高危组。低危组CD34(+)细胞凋亡率较其他组显著升高（p < 0.05）。高危MDS组骨髓活检MVD显著升高（p < 0.05），低危MDS组MVD也高于对照组（p < 0.05）。低危组CD34(+)细胞凋亡率与SDF-1水平呈正相关，高危组SDF-1水平与MVD呈正相关。结论：MDS患者骨髓中SDF-1表达、CD34(+)细胞凋亡率及MVD均显著异常，尤其在不同危险组中，提示SDF-1水平与细胞凋亡及血管生成有关。
