[基质细胞衍生因子-1/CXCR4生物学轴在骨髓增生异常综合征中的表达]

Zhang Yi-zhuo, Da Wan-ming, Zhao Dan-dan, Zhao Hai-feng, Wu Xiao-xiong, Wang Hui

Department of Hematology, Tianjin Medical University Cancer Hospital, Tianjin, China.

Zhonghua Yi Xue Za Zhi. 2011 Dec 13;91(46):3275-7.

OBJECTIVE

To explore the expression of stromal cell derived factor-1 (SDF-1) and its receptor CXCR4 in myelodysplastic syndromes (MDS).

METHODS

A total of 59 patients with a diagnosis of MDS were divided into low-grade (n = 33) and high-grade (n = 26) groups according to international prognostic scoring system (IPSS). Bone marrow (BM) samples were collected. The SDF-1 and VEGF levels in BM plasma, CXCR4 expression on BM CD34(+) cell and the apoptosis of CD34(+) cells were measured.

RESULTS

The SDF-1 levels in MDS patients were significantly higher than those of normal controls [(689 ± 190) ng/L, P < 0.05]. And the low-grade group was significantly higher than that of high-grade group [(2301 ± 413) vs (1173 ± 501) ng/L]. CXCR4 expression on CD34(+) cells were significantly higher in high-grade group (68.1% ± 18.8%) than that of both low-grade (21.0% ± 9.7%) and control groups (19.4% ± 5.3%) (P < 0.05). Apoptotic rate of CD34(+) cells were 54.8% ± 10.2% in low-grade group, 24.3% ± 7.9% in high-grade group and 18.5% ± 8.7% in control group. It significantly increased in low-grade group versus other groups (P < 0.05). VEGF levels were significantly higher in MDS patients in low-grade group [(286 ± 97) ng/L] and high-grade group [(407 ± 168) ng/L] versus control group [(157 ± 46) ng/L, P < 0.05]. A positive correlation was found between apoptosis of CD34(+) cells and SDF-1 levels in low-grade group (r = 0.805, P < 0.05), VEGF levels and CXCR4 expression rate in high-grade group (r = 0.683, P < 0.05).

CONCLUSION

The expression of SDF-1/CXCR4 is significantly abnormal in MDS patients. And it is correlated with apoptosis and angiogenesis. Intervention of SDF-1/CXCR4 axis may provide a new therapeutic target.

目的

探讨基质细胞衍生因子-1（SDF-1）及其受体CXCR4在骨髓增生异常综合征（MDS）中的表达情况。

方法

59例确诊为MDS的患者根据国际预后评分系统（IPSS）分为低危组（n = 33）和高危组（n = 26）。采集骨髓（BM）样本。检测BM血浆中SDF-1和VEGF水平、BM CD34(+)细胞上CXCR4的表达以及CD34(+)细胞的凋亡情况。

结果

MDS患者的SDF-1水平显著高于正常对照组[（689±190）ng/L，P < 0.05]。且低危组显著高于高危组[（2301±413）对（1173±501）ng/L]。高危组CD34(+)细胞上CXCR4的表达（68.1%±18.8%）显著高于低危组（21.0%±9.7%）和对照组（19.4%±5.3%）（P < 0.05）。低危组CD34(+)细胞的凋亡率为54.8%±10.2%，高危组为24.3%±7.9%，对照组为18.5%±8.7%。低危组与其他组相比凋亡率显著升高（P < 0.05）。MDS患者低危组[（286±97）ng/L]和高危组[（407±168）ng/L]的VEGF水平显著高于对照组[（157±46）ng/L，P < 0.05]。低危组CD34(+)细胞凋亡与SDF-1水平呈正相关（r = 0.805，P < 0.05），高危组VEGF水平与CXCR4表达率呈正相关（r = 0.683，P < 0.05）。