Department of Cadiothoracic, The Second Xiangya Hospital Affiliated with Central-South University, No. 139A, Changsha, Hunan, China.
Cell Biochem Biophys. 2012 Jan;62(1):101-5. doi: 10.1007/s12013-011-9266-5.
We sought to compare the preventive effects of mitomycin-C(MMC) and chitosan on intraarticular adhesion after knee surgery in rabbits. For this purpose, 48 New-Zealand rabbits were randomly and equally divided into MMC, chitosan, and control groups. Approximately 10 × 10 mm(2) of the cortical bone was removed from both sides of left femoral condyle and the cancellous bone underneath was exposed. The decorticated areas were topically treated with MMC and chitosan while control group was treated with physiological saline. The lower left limb was fixed in flexed position with Kirschner-wire for 4 weeks postoperatively. After 4 weeks, gross and histopathological examination, biochemical analysis, and fibroblast counts were performed on knee intraarticular adhesion in each group. The data show mild membrane-like fibrous intraarticular adhesion, presented in loose, in MMC group. There was moderate intraarticular adhesion in chitosan group while in controls; there was large-size compact fibrous tissue adhesion. Hydroxyproline contents and fibroblast quantity of MMC and chitosan groups were lower (P < 0.05) than that of control group. We, therefore, concluded that MMC and chitosan could prevent intraarticular adhesion of the knee in rabbits by inhibiting fibroblast proliferation and reducing collagenous fiber formation while MMC had a better preventive effect than that of chitosan.
我们旨在比较丝裂霉素 C（MMC）和壳聚糖对兔膝关节手术后关节内粘连的预防作用。为此，将 48 只新西兰兔随机均分为 MMC 组、壳聚糖组和对照组。从左侧股骨髁的两侧去除约 10×10mm²的皮质骨，暴露下方的松质骨。脱钙区域用 MMC 和壳聚糖局部处理，而对照组用生理盐水处理。术后第 4 周，将左下肢用克氏针固定在弯曲位置。4 周后，对各组膝关节关节内粘连进行大体和组织病理学检查、生化分析和成纤维细胞计数。数据显示，MMC 组表现为轻度膜状纤维性关节内粘连，呈疏松状；壳聚糖组为中度关节内粘连；对照组则为大尺寸致密纤维组织粘连。MMC 和壳聚糖组羟脯氨酸含量和成纤维细胞数量均低于对照组（P<0.05）。因此，我们得出结论，MMC 和壳聚糖可通过抑制成纤维细胞增殖和减少胶原纤维形成来预防兔膝关节关节内粘连，且 MMC 的预防效果优于壳聚糖。
