Liang Yuan, Sun Yu, Li Xiaolei, Yan Lianqi, Wang Jingcheng, Hu Jinlong, Yu Huan, Xiao Haixiang, Chen Hui, Sun Zhongwei, Cai Jun, Feng Xinmin, Xiong Chuanzhi, He Jinshan
1] Department of Orthopedics, Clinical Medical College of Yangzhou University, Subei People's Hospital, Nantong West Road 98, Yangzhou, 225001, China [2].
Department of Orthopedics, Clinical Medical College of Yangzhou University, Subei People's Hospital, Nantong West Road 98, Yangzhou, 225001, China.
Sci Rep. 2014 Apr 9;4:4621. doi: 10.1038/srep04621.
10-Hydroxycamptothecin could reduce intraarticular adhesion by inhibiting fibroblasts proliferation after knee surgery. However, the ideal concentration of hydroxycamptothecin have not been defined. This study was tried to verify the optimal concentration of 10-hydroxycamptothecin in preventing knee intraarticular adhesion. Sixty rabbits were randomly divided into five groups. Approximately 10 mm × 10 mm of the cortical bone was removed from both sides of the femoral condyle and the underneath cancellous bone was exposed. Various concentrations of hydroxycamptothecin (0.1 mg/ml, 0.5 mg/ml, 1.0 mg/ml, 2.0 mg/ml) or saline were applied to the decorticated areas for 10 minutes. After four weeks, the degree of inraarticular adhesion was assessed by macroscopic evaluation, biochemical analysis of hydroxyproline content and histological evaluation. The results demonstrated that the extent of knee inraarticular adhesion in 1.0 mg/ml group and 2.0 mg/ml hydroxycamptothecin group were significantly lower than those of 0.5 mg/ml group, 0.1 mg/ml hydroxycamptothecin group and control group. Moreover, there was no significant difference between 1.0 mg/ml group and 2.0 mg/ml hydroxycamptothecin group. In conclusion, topical application of 1.0 mg/ml hydroxycamptothecin may be the optimal concentration in reducing intraarticular adhesion after knee surgery in rabbits.
10-羟基喜树碱可通过抑制膝关节手术后成纤维细胞增殖来减少关节内粘连。然而，喜树碱的理想浓度尚未确定。本研究试图验证10-羟基喜树碱预防兔膝关节内粘连的最佳浓度。60只家兔随机分为五组。从股骨髁两侧去除约10 mm×10 mm的皮质骨，暴露下方的松质骨。将不同浓度的喜树碱（0.1 mg/ml、0.5 mg/ml、1.0 mg/ml、2.0 mg/ml）或生理盐水应用于去皮质区域10分钟。四周后，通过宏观评估、羟脯氨酸含量的生化分析和组织学评估来评估关节内粘连程度。结果表明，1.0 mg/ml组和2.0 mg/ml喜树碱组的膝关节内粘连程度明显低于0.5 mg/ml组、0.1 mg/ml喜树碱组和对照组。此外，1.0 mg/ml组和2.0 mg/ml喜树碱组之间无显著差异。总之，局部应用1.0 mg/ml喜树碱可能是减少兔膝关节手术后关节内粘连的最佳浓度。
