Biophysics Unit, Manipal Life Sciences Centre, Manipal University, Manipal 576104, Karnataka, India.
J Biophotonics. 2012 Feb;5(2):168-84. doi: 10.1002/jbio.201100089. Epub 2011 Dec 15.
The present study focuses on the evaluation of the effect of He-Ne laser on tissue regeneration by monitoring collagen synthesis in wound granulation tissues in Swiss albino mice using analysis of laser induced fluorescence (LIF) and light microscopy techniques. The spectral analyses of the wound granulation tissues have indicated a dose dependent increase in collagen levels during the post-wounding days. The histological examinations on the other hand have also shown a significant increase in collagen deposition along with the reduced edema, leukocytes, increased granulation tissue, and fibroblast number in the optimal laser dose treated group compared to the non-illuminated controls.
本研究通过激光诱导荧光（LIF）和光学显微镜技术分析，观察瑞士白化小鼠伤口肉芽组织中胶原蛋白的合成，评估氦氖激光对组织再生的影响。光谱分析表明，在创伤后几天内，胶原蛋白水平呈剂量依赖性增加。另一方面，组织学检查也表明，与未照射对照组相比，最佳激光剂量处理组的胶原沉积显著增加，同时水肿、白细胞减少、肉芽组织增加和成纤维细胞数量增加。
