使用双光子显微镜对局部低剂量光动力疗法在伤口愈合中的有效性进行体内定量分析。
In Vivo Quantification of the Effectiveness of Topical Low-Dose Photodynamic Therapy in Wound Healing Using Two-Photon Microscopy.
作者信息
Zuhayri Hala, Nikolaev Viktor V, Knyazkova Anastasia I, Lepekhina Tatiana B, Krivova Natalya A, Tuchin Valery V, Kistenev Yury V
机构信息
Laboratory of Laser Molecular Imaging and Machine Learning, Tomsk State University, 36 Lenin Av., 634050 Tomsk, Russia.
Science Medical Center, Saratov State University, 83 Astrakhanskaya Str., 410012 Saratov, Russia.
出版信息
Pharmaceutics. 2022 Jan 26;14(2):287. doi: 10.3390/pharmaceutics14020287.
The effect of low-dose photodynamic therapy on in vivo wound healing with topical application of 5-aminolevulinic acid and methylene blue was investigated using an animal model for two laser radiation doses (1 and 4 J/cm). A second-harmonic-generation-to-auto-fluorescence aging index of the dermis (SAAID) was analyzed by two-photon microscopy. SAAID measured at 60-80 μm depths was shown to be a suitable quantitative parameter to monitor wound healing. A comparison of SAAID in healthy and wound tissues during phototherapy showed that both light doses were effective for wound healing; however, healing was better at a dose of 4 J/cm.
使用动物模型，研究了低剂量光动力疗法通过局部应用5-氨基乙酰丙酸和亚甲蓝对体内伤口愈合的影响，采用了两种激光辐射剂量（1和4 J/cm²）。通过双光子显微镜分析了真皮的二次谐波产生与自发荧光老化指数（SAAID）。结果表明，在60-80μm深度处测量的SAAID是监测伤口愈合的合适定量参数。光疗期间健康组织和伤口组织中SAAID的比较表明，两种光剂量对伤口愈合均有效；然而，4 J/cm²剂量的愈合效果更好。
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