Yamauchi Yasuyuki, Yagi Hiromichi, Usui Yoshihiko, Kimura Keisuke, Agawa Tsuyoshi, Tsukahara Rintaro, Yamakawa Naoyuki, Goto Hiroshi
Department of Ophthalmology, Tokyo Medical University Hospital, Tokyo, Japan.
Clin Ophthalmol. 2011;5:1649-53. doi: 10.2147/OPTH.S26661. Epub 2011 Nov 22.
Recent research on macular diseases has prompted investigations into the condition of the intersection between the photoreceptor inner and outer segments (IS/OS) and the relationship with retinal photoreceptor abnormalities. Although the origin of the IS/OS in optical coherence tomography (OCT) images is unclear, it may be related to either the cellular activity of the photoreceptors or the structure of the OS disks. To address this question, we compared the IS/OS status in OCT images of rat retinas before and after euthanasia.
OCT images were taken before and after euthanasia in four eyes of two Brown Norway rats. After the OCT images were taken, the rats were used for histopathological studies to confirm that retinal structures were intact.
Before euthanasia, the IS/OS and external limiting membrane (ELM) line were clearly identifiable on the OCT images. However, after euthanasia, neither the IS/OS nor the ELM line was evident in three out of four eyes, and a faint IS/OS and an ELM line were identified in one eye. Histopathological analysis did not show any abnormalities in the retina in any of the four eyes.
The origin of the IS/OS identified in OCT images is likely related to the biological activities of the photoreceptor cells.
近期关于黄斑疾病的研究促使人们对光感受器内节与外节（IS/OS）交界处的情况及其与视网膜光感受器异常的关系展开调查。尽管光学相干断层扫描（OCT）图像中IS/OS的起源尚不清楚，但它可能与光感受器的细胞活动或外节盘膜的结构有关。为解决这个问题，我们比较了安乐死前后大鼠视网膜OCT图像中的IS/OS状态。
对两只棕色挪威大鼠的四只眼睛在安乐死前后进行了OCT成像。在拍摄OCT图像后，将大鼠用于组织病理学研究，以确认视网膜结构完整。
在安乐死之前，OCT图像上可清晰识别出IS/OS和外界膜（ELM）线。然而，在安乐死后，四只眼睛中有三只眼睛的IS/OS和ELM线均不明显，只有一只眼睛中识别出了模糊的IS/OS和ELM线。组织病理学分析显示，四只眼睛的视网膜均未出现任何异常。
OCT图像中识别出的IS/OS起源可能与光感受器细胞的生物活性有关。
