Yao Lei, Wang Zhenyu, Zhao Haitian, Cheng Cuilin, Fu Xiaoyi, Liu Jiaren, Yang Xin
School of Food Science and Engineering, Harbin Institute of Technology, 73 HuangHe Road, NanGang District, Harbin 150090, China; E-Mails:
Int J Mol Sci. 2011;12(11):8096-104. doi: 10.3390/ijms12118096. Epub 2011 Nov 17.
The aim of this study was to investigate radioprotective effect of the polysaccharides from soybean meal (SMP) against X-ray radiation-induced damage in mouse spleen lymphocytes. MTT and comet assay were performed to evaluate SMP's ability to prevent cell death and DNA damage induced by radiation. The results show that, X-ray radiation (30 KV, 10 mA, 8 min (4 Gy)) can significantly increase cell death and DNA fragmentation of mouse spleen lymphocytes. Pretreatment with SMP for 2 h before radiation could increase cell viability, moreover, the SMP can reduce X-ray radiation-induced DNA damage. The percentage of tail DNA and the tail moment of the SMP groups were significantly lower than those of the radiation alone group (p < 0.05). These results suggest SMP may be a good candidate as a radioprotective agent.
本研究旨在探讨豆粕多糖（SMP）对X射线辐射诱导的小鼠脾脏淋巴细胞损伤的辐射防护作用。采用MTT法和彗星试验评估SMP预防辐射诱导的细胞死亡和DNA损伤的能力。结果表明，X射线辐射（30 kV，10 mA，8分钟（4 Gy））可显著增加小鼠脾脏淋巴细胞的细胞死亡和DNA片段化。辐射前用SMP预处理2小时可提高细胞活力，此外，SMP可减少X射线辐射诱导的DNA损伤。SMP组的尾DNA百分比和尾矩显著低于单纯辐射组（p < 0.05）。这些结果表明SMP可能是一种良好的辐射防护剂候选物。
