Yan Xiu-Feng, Gao Shuang, Xia Ju-Feng, Ye Rong, Yu Hui, Long Jian-Er
Laboratory of Medical Microbiology, Department of Medical Microbiology and Parasitology, Shanghai Medical College of Fudan University, Shanghai, China.
Scand J Infect Dis. 2012 Apr;44(4):297-305. doi: 10.3109/00365548.2011.634433. Epub 2011 Dec 18.
Enterovirus 71 (EV71) has been the main causative agent of hand, foot, and mouth disease (HFMD) outbreaks in recent years. A significant increase in the number of HFMD cases in China over the last 3 y has made the public prevention and therapy of this disease a critical issue.
A total of 3208 HFMD patients in Shanghai during the period 2009 to 2010 were analyzed; 437 clinical specimens were collected for the determination of causative pathogens. Eight of the isolated EV71 strains were sequenced and phylogenetically analyzed.
The widespread outbreak of HFMD in Shanghai was caused predominantly by EV71 (86.5%), and in part by coxsackievirus A16 (CA16) (6.9%). The high incidence of mixed infections with EV71 and CA16 (17.6% of the total CA16-infected cases) has never before been observed in China. Most HFMD patients (76.9%) were aged 1-4 y. Boys showed a higher HFMD prevalence rate (65.3%) than girls (34.7%). Phylogenetic analysis on the basis of the VP1 gene and the complete genome sequences revealed that the EV71 strains that circulated in Shanghai belonged to the C4 subgenotype.
EV71 subgenotype C4 was the major causative agent of the HFMD outbreak in Shanghai. A high incidence of mixed infections with EV71 and CA16 was also observed.
肠道病毒71型(EV71)是近年来手足口病(HFMD)疫情的主要病原体。过去3年中国手足口病病例数显著增加,使得该病的公共预防和治疗成为关键问题。
对2009年至2010年期间上海的3208例手足口病患者进行分析;收集437份临床标本以确定病原体。对分离出的8株EV71毒株进行测序并进行系统发育分析。
上海手足口病的广泛暴发主要由EV71引起(86.5%),部分由柯萨奇病毒A16型(CA16)引起(6.9%)。EV71和CA16混合感染的高发生率(占CA16感染病例总数的17.6%)在中国此前从未被观察到。大多数手足口病患者(76.9%)年龄在1至4岁。男孩手足口病患病率(65.3%)高于女孩(34.7%)。基于VP1基因和全基因组序列的系统发育分析表明,在上海流行的EV71毒株属于C4亚型。
EV71 C4亚型是上海手足口病暴发的主要病原体。还观察到EV71和CA16混合感染的高发生率。