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J Hazard Mater. 2012 Jan 30;201-202:236-43. doi: 10.1016/j.jhazmat.2011.11.074. Epub 2011 Nov 28.
Ethyl tert-butyl ether (ETBE) was detected at high concentration (300mgL(-1)) in the groundwater below a gas-station. No significant carbon neither hydrogen isotopic fractionation of ETBE was detected along the plume. ETBE and BTEX biodegradation capacities of the indigenous microflora Pz1-ETBE and of a culture (MC-IFP) composed of Rhodococcus wratislaviensis IFP 2016, Rhodococcus aetherivorans IFP 2017 and Aquincola tertiaricarbonis IFP 2003 showed that ETBE and BTEX degradation rates were in the same range (ETBE: 0.91 and 0.83 mg L(-1)h(-1) and BTEX: 0.64 and 0.82 mg L(-1)h(-1), respectively) but tert-butanol (TBA) accumulated transiently at a high level using Pz1-ETBE (74 mg L(-1)). An on-site pilot plant (2m(3)) filled with polluted groundwater and inoculated by MC-IFP, successfully degraded four successive additions of ETBE and gasoline. However, an insignificant ETBE isotopic fractionation was also accompanying this decrease which suggested the involvement of low fractionating-strains using EthB enzymes, but required of additional proofs. The ethB gene encoding a cytochrome P450 involved in ETBE biodegradation (present in R. aetherivorans IFP 2017) was monitored by quantitative real-time polymerase chain reaction (q-PCR) on DNA extracted from water sampled in the pilot plant which yield up to 5×10(6) copies of ethB gene per L(-1).
乙基叔丁基醚（ETBE）在一个加油站下方的地下水层中被检测到高浓度（300mgL(-1)）。沿着羽流，未检测到 ETBE 的显著碳和氢同位素分馏。土著微生物 Pz1-ETBE 和由 Rhodococcus wratislaviensis IFP 2016、Rhodococcus aetherivorans IFP 2017 和 Aquincola tertiaricarbonis IFP 2003 组成的培养物（MC-IFP）的 ETBE 和 BTEX 生物降解能力表明，ETBE 和 BTEX 的降解速率在同一范围内（ETBE：0.91 和 0.83mgL(-1)h(-1)和 BTEX：0.64 和 0.82mgL(-1)h(-1)），但使用 Pz1-ETBE 会短暂地积累高水平的叔丁醇（TBA）（74mgL(-1)）。一个装满受污染地下水并用 MC-IFP 接种的现场中试工厂（2m(3)）成功地降解了连续四次添加的 ETBE 和汽油。然而，这种减少伴随着 ETBE 的同位素分馏不明显，这表明涉及使用 EthB 酶的低分馏菌株，但需要额外的证明。通过定量实时聚合酶链反应（q-PCR）监测参与 ETBE 生物降解的细胞色素 P450 编码基因（存在于 Rhodococcus aetherivorans IFP 2017 中）从中试工厂采集的水样中提取的 DNA，该方法可产生高达 5×10(6)拷贝的 ethB 基因/L(-1)。
