IFP, Rueil-Malmaison, France.
FEMS Microbiol Ecol. 2010 May;72(2):289-96. doi: 10.1111/j.1574-6941.2010.00847.x. Epub 2010 Feb 11.
The degradation of fuel oxygenates [methyl tert-butyl ether (MTBE), ethyl tert-butyl ether (ETBE) and tert-amyl methyl ether (TAME)] by Rhodococcus ruber IFP 2001, Rhodococcus zopfii IFP 2005 and Gordonia sp. IFP 2009 (formerly Mycobacterium sp.) isolated from different environments was compared. Strains IFP 2001, IFP 2005 and IFP 2009 grew on ETBE due in part to the activity of a cytochrome P450, CYP249. All of these strains were able to degrade ETBE to tert-butyl alcohol and are harboring the CYP249 cytochrome P450. They were also able to degrade MTBE and TAME, but ETBE was degraded in all cases most efficiently, with degradation rates measured after growth on ETBE of 2.1, 3.5 and 1.6 mmol ETBE g(-1) dry weight h(-1) for strains IFP 2001, IFP 2005 and IFP 2009, respectively. The phylogenetic relationships between the different ethR (encoding the regulator) and ethB (encoding the cytochrome P450) genes were determined and showed high identity between different ethB genes (>99%). Only ETBE was able to induce the expression of ethB in strains IFP 2001 and IFP 2005 as measured by reverse transcriptase quantitative PCR. Our results are a first indication of the possible role played by the ethB gene in the ecology of ETBE degradation.
从不同环境中分离到的红球菌（Rhodococcus ruber IFP 2001）、小诺卡氏菌（Rhodococcus zopfii IFP 2005）和戈登氏菌（Gordonia sp. IFP 2009）（原分枝杆菌属）对燃料含氧化合物[甲基叔丁基醚（MTBE）、乙基叔丁基醚（ETBE）和叔戊基甲基醚（TAME）]的降解能力进行了比较。IFP 2001、IFP 2005 和 IFP 2009 菌株能够利用 ETBE 生长，部分原因是细胞色素 P450（CYP249）的活性。所有这些菌株都能够将 ETBE 降解为叔丁醇，并携带 CYP249 细胞色素 P450。它们还能够降解 MTBE 和 TAME，但在所有情况下，ETBE 的降解效率最高，在以 ETBE 为唯一碳源生长后，菌株 IFP 2001、IFP 2005 和 IFP 2009 的 ETBE 降解速率分别为 2.1、3.5 和 1.6mmol ETBE g(-1) 干重 h(-1)。不同 ethR（编码调控因子）和 ethB（编码细胞色素 P450）基因之间的系统发育关系已经确定，不同 ethB 基因之间具有高度的同源性（>99%）。只有 ETBE 能够诱导 IFP 2001 和 IFP 2005 菌株中 ethB 的表达，这是通过逆转录定量 PCR 测量的。我们的结果首次表明 ethB 基因在 ETBE 降解生态中的可能作用。
