Department of Biosciences, Jamia Millia Islamia, New Delhi-110025, India.
Fitoterapia. 2012 Apr;83(3):434-40. doi: 10.1016/j.fitote.2011.12.003. Epub 2011 Dec 9.
Cinnamaldehyde, its derivatives and curcumin are reported to have strong antifungal activity. In this work we report and compare anticandidal activity of curcumin (CUR) and α-methyl cinnamaldehyde (MCD) against 38 strains of Candida (3; standard, fluconazole sensitive, 24; clinical, fluconazole sensitive, 11; clinical, fluconazole resistant). The minimum inhibitory concentrations (MIC₉₀) of CUR ranged from 250 to 650 μg/ml for sensitive strains and from 250 to 500 μg/ml for resistant strains. MIC₉₀ of MCD varied between 100 and 250 μg/ml and 100-200 μg/ml for sensitive and resistant strains, respectively. Higher activity of MCD as compared to CUR was further reinforced by spot assays and growth curve studies. At their respective MIC₉₀ values, in the presence of glucose, average inhibition of H+-efflux caused by CUR and MCD against standard, clinical and resistant isolates was 24%, 31%, 32% and 54%, 52%, 54%, respectively. Inhibition of H+-extrusion leads to intracellular acidification and cell death, average pHi for control, CUR and MCD exposed cells was 6.68, 6.39 and 6.20, respectively. Scanning electron micrographs of treated cells show more extensive damage in case of MCD. Haemolytic activity of CUR and MCD at their highest MIC was 11.45% and 13.00%, respectively as against 20% shown by fluconazole at typical MIC of 30 μg/ml. In conclusion, this study shows significant anticandidal activity of CUR and MCD against both azole-resistant and sensitive clinical isolates, MCD is found to be more effective.
肉桂醛、其衍生物和姜黄素据报道具有很强的抗真菌活性。在这项工作中，我们报告并比较了姜黄素（CUR）和α-甲基肉桂醛（MCD）对 38 株念珠菌（3 株；标准，氟康唑敏感，24 株；临床，氟康唑敏感，11 株；临床，氟康唑耐药）的抗真菌活性。CUR 的最小抑菌浓度（MIC₉₀）范围为 250 至 650 μg/ml，适用于敏感株，250 至 500 μg/ml，适用于耐药株。MCD 的 MIC₉₀ 分别在 100 至 250 μg/ml 和 100-200 μg/ml 之间，适用于敏感株和耐药株。与 CUR 相比，MCD 的更高活性进一步通过斑点分析和生长曲线研究得到了加强。在各自的 MIC₉₀ 值下，在葡萄糖存在下，CUR 和 MCD 对标准、临床和耐药分离株的 H+-外排抑制的平均抑制率分别为 24%、31%、32%和 54%、52%、54%。H+-外排的抑制导致细胞内酸化和细胞死亡，对照、CUR 和 MCD 暴露细胞的平均 pHi 分别为 6.68、6.39 和 6.20。处理细胞的扫描电子显微镜照片显示，MCD 情况下的损伤更为广泛。CUR 和 MCD 在最高 MIC 时的溶血活性分别为 11.45%和 13.00%，而氟康唑在典型 MIC 为 30 μg/ml 时的溶血活性为 20%。总之，这项研究表明 CUR 和 MCD 对唑类耐药和敏感的临床分离株均具有显著的抗真菌活性，MCD 更为有效。
