Department of Biosciences, Jamia Millia Islamia, New Delhi, 110025, India.
Fitoterapia. 2011 Oct;82(7):1012-20. doi: 10.1016/j.fitote.2011.06.004. Epub 2011 Jun 25.
Fluconazole resistance is becoming an important clinical concern. We studied the in vitro effects of cinnamaldehyde against 18 fluconazole-resistant Candida isolates. MIC(90) of cinnamaldehyde against different Candida isolates ranged 100-500 μg/ml. Growth and sensitivity of the organisms were significantly affected by cinnamaldehyde at different concentrations. The rapid irreversible action of this compound on fungal cells suggested membrane-located targets for its action. Insight studies to mechanism suggested that cinnamaldehyde exerts its antifungal activity by targeting sterol biosynthesis and plasma membrane ATPase activity. Inhibition of H(+) (-)ATPase leads to intracellular acidification and cell death. Toxicity against H9c2 rat cardiac myoblasts was studied to exclude the possibility of further associated cytotoxicity. The observed selectively fungicidal characteristics against fluconazole-resistant Candida isolates signify a promising candidature of this essential oil as an antifungal agent in treatments for candidosis.
氟康唑耐药性正成为一个重要的临床关注点。我们研究了肉桂醛对 18 株氟康唑耐药性念珠菌分离株的体外作用。肉桂醛对不同念珠菌分离株的 MIC(90)范围为 100-500μg/ml。肉桂醛在不同浓度下对生物的生长和敏感性有显著影响。该化合物对真菌细胞的快速不可逆作用表明其作用的靶位位于膜上。对机制的深入研究表明，肉桂醛通过靶向甾醇生物合成和质膜 ATP 酶活性发挥其抗真菌活性。抑制 H(+) (-)ATP 酶导致细胞内酸化和细胞死亡。为排除进一步相关细胞毒性的可能性，研究了肉桂醛对 H9c2 大鼠心肌成肌细胞的毒性。对氟康唑耐药性念珠菌分离株的选择性杀菌特性表明，这种精油作为抗真菌剂治疗念珠菌病具有很大的应用潜力。
