Department of Urology, The Fourth Affiliated Hospital of Nantong Medical College, Yancheng City No 1 People's Hospital, Nanjing, Jiangsu, PR China.
Mol Med Rep. 2012 Mar;5(3):695-9. doi: 10.3892/mmr.2011.711. Epub 2011 Dec 14.
Dysregulation of microRNA metabolism has been observed in a variety of human cancers. In this study, we evaluated the expression of the enzymes of the machinery Dicer, Drosha and DGCR8, in transitional cell carcinomas (TCCs) of the urinary bladder. The expression of Dicer, Drosha and DGCR8 was analyzed using semi-quantitative RT-PCR in clinical specimens from normal bladder mucosa, TCCs and their normal adjacent tissues (NATs). Immunohistochemistry was performed to compare the expression of Dicer in normal, TCCs and NATs. Our study demonstrated that Dicer mRNA levels in TCCs were significantly lower compared to normal samples and NAT samples. The immunohistochemistry results revealed that Dicer protein levels in TCCs were significantly downregulated compared to normal bladder mucosa and NATs. Our data demonstrated that Dicer is significantly downregulated in TCCs compared to paired NAT samples and normal samples, suggesting that reduced expression of Dicer may play an important role in bladder cancer.
微 RNA 代谢失调已在多种人类癌症中被观察到。在这项研究中，我们评估了 Dicer、Drosha 和 DGCR8 酶在膀胱移行细胞癌 (TCC) 中的表达。使用半定量 RT-PCR 在来自正常膀胱黏膜、TCC 和其正常相邻组织 (NAT) 的临床标本中分析 Dicer、Drosha 和 DGCR8 的表达。免疫组织化学用于比较 Dicer 在正常、TCC 和 NAT 中的表达。我们的研究表明，与正常样本和 NAT 样本相比，TCC 中的 Dicer mRNA 水平显著降低。免疫组织化学结果显示，与正常膀胱黏膜和 NAT 相比，TCC 中的 Dicer 蛋白水平显著下调。我们的数据表明，与配对的 NAT 样本和正常样本相比，Dicer 在 TCC 中显著下调，提示 Dicer 表达减少可能在膀胱癌中发挥重要作用。
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