Rahimi Giti, Jafari Naser, Khodabakhsh Mehnoush, Shirzad Zohreh, Dogaheh Hadi Peeri
Department of Obstetrics and Gynecology .
Gynecol Endocrinol. 2015 Feb;31(2):156-9. doi: 10.3109/09513590.2014.969700. Epub 2014 Oct 8.
MicroRNAs (miRNAs) have been shown to play important roles in diverse cellular processes and linked to variety of disorders. Dicer and Drosha are two major enzymes in the miRNA biogenesis process. DGCR8 is the assistant of Drosha in the microprocessor complex. In this study, we evaluated the mRNA expression profiles of major miRNA processing machinery Drosha, Dicer, and DGCR8 in gestational diabetes mellitus (GDM), pregnant and healthy women. Our findings indicate that the expression levels of Drosha, Dicer and DGCR8 were upregulated in both pregnant and GDM patients compared to the control group. However, Drosha and Dicer were upregulated more than pregnant group. In conclusion, we detected dysregulation of Drosha, Dicer and DGCR8 expression in pregnant and GDM patients when compared to healthy control participants. Therefore, we favor the hypothesis that miRNAs are involved in the development of GDM.
微小RNA（miRNA）已被证明在多种细胞过程中发挥重要作用，并与多种疾病相关联。Dicer和Drosha是miRNA生物合成过程中的两种主要酶。DGCR8是微处理器复合物中Drosha的辅助因子。在本研究中，我们评估了妊娠糖尿病（GDM）患者、孕妇和健康女性中主要miRNA加工机制Drosha、Dicer和DGCR8的mRNA表达谱。我们的研究结果表明，与对照组相比，孕妇和GDM患者中Drosha、Dicer和DGCR8的表达水平均上调。然而，Drosha和Dicer的上调幅度大于孕妇组。总之，与健康对照参与者相比，我们检测到孕妇和GDM患者中Drosha、Dicer和DGCR8表达失调。因此，我们支持miRNA参与GDM发生发展的假说。
