Institute of Cellular and Molecular Biology, College of Life Science, Xuzhou Normal University, China.
Biochem Genet. 2011 Aug;49(7-8):533-9. doi: 10.1007/s10528-011-9428-6. Epub 2011 Mar 3.
Acrp30 plays a critical role in the regulation of glucose and lipid homeostasis. In this study, polymorphism of the Acrp30 gene was detected by PCR-SSCP and DNA sequencing methods in 321 individuals from three goat breeds, and the association of Acrp30 gene polymorphism with growth traits in the three goat breeds was analyzed. A novel insert/deletion (GT)(5) microsatellite sequence was detected in the 5' flanking region of the gene. Three genotypes (AA, AB, and BB) were found in three breeds. There was moderate genetic diversity in the locus in the analyzed populations. Significant associations were observed between the genotypes of the locus and growth traits in the Boer goat population. The chest circumference of individuals with genotype BB was significantly greater than that of individuals with genotype AA.
Acrp30 在调节葡萄糖和脂质稳态方面发挥着关键作用。本研究采用 PCR-SSCP 和 DNA 测序方法检测了来自三个山羊品种的 321 个人的 Acrp30 基因多态性，并分析了 Acrp30 基因多态性与三个山羊品种生长性状的关系。在基因的 5' 侧翼区检测到一个新的插入/缺失（GT）（5）微卫星序列。在三个品种中发现了三种基因型（AA、AB 和 BB）。在分析的群体中，该位点具有中度遗传多样性。在波尔山羊群体中，该位点的基因型与生长性状之间存在显著关联。基因型为 BB 的个体的胸围显著大于基因型为 AA 的个体。
