Xu W, Qi G, Liu Y
Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing.
Zhonghua Liu Xing Bing Xue Za Zhi. 1994 Aug;15(4):218-22.
Multilocus enzyme analysis by polyacrylamide gel electrophoresis (PAGE) was used to analyse 335 isolates of S. typhi from several provinces where high morbidity of typhoid fever was observed in recent years. We analysed 7 metabolic enzymes encoded by chromosome. The results showed that all the enzyme loci were polymorphic. The average alleles per locus was 7.4 (2-19): the mean genetic diversity per locus was 0.498 (0.198 0.883). There were 147 distinctive electrophoretic types (ETs) in 335 isolates. With cluster analysis by the average-linkage method, we found that there were 22 subclones, 12 clones among all isolates studied. Most isolates in 1988 from both Jiangsu and Zhejiang provinces belonged to one clone; the isolates from Guangxi Province in 1990 and parts of Hubei isolates belonged to one clone; the isolates from Hunan and Guizhou provinces during several-year period were homogeneous. However, the isolates from Xinjiang Autonomous Region were remarkebly different from other provinces, belonging to a particular clone. We also observed that the isolates from an outbreak or epidemic were from a one subclone.
采用聚丙烯酰胺凝胶电泳（PAGE）进行多位点酶分析，以分析近年来伤寒发病率较高的几个省份的335株伤寒沙门氏菌分离株。我们分析了由染色体编码的7种代谢酶。结果表明，所有酶位点均具有多态性。每个位点的平均等位基因数为7.4（2 - 19）：每个位点的平均遗传多样性为0.498（0.198 - 0.883）。335株分离株中有147种独特的电泳类型（ETs）。通过平均连锁法进行聚类分析，我们发现所有研究的分离株中有22个亚克隆、12个克隆。1988年来自江苏和浙江的大多数分离株属于一个克隆；1990年来自广西的分离株和部分湖北分离株属于一个克隆；几年间来自湖南和贵州的分离株具有同质性。然而，来自新疆自治区的分离株与其他省份明显不同，属于一个特定的克隆。我们还观察到，来自暴发或流行的分离株来自一个亚克隆。
