Agostoni E, Zocchi L
Istituto di Fisiologia Umana I, Università di Milano, Italy.
Respir Physiol. 1990 Jul;81(1):19-27. doi: 10.1016/0034-5687(90)90066-8.
The occurrence of a solute-coupled absorption of liquid from the pleural space was studied by measuring in anesthetized rabbits the volume of liquid of the right pleural space 1 h after injecting into it 2 ml of Ringer solution or of Ringer with an inhibitor of a Na(+)-Cl- coupled transport or of the Na+/K+ pump. Volume collected after Ringer was 1.56 +/- 0.08 ml. Initial volume being 2.2 ml, net absorption rate was 0.64 ml/h. Volume collected after disulfonic stilbene (0.1 mM) or bumetanide (0.1 mM) was 2.01 +/- 0.06 and 2.01 +/- 0.05 ml, respectively; net absorption rate was reduced to 0.19 ml/h. This suggests the occurrence of Na(+)-Cl- coupled transports. Volume collected after ouabain (0.5 mM) was 2.08 +/- 0.08 ml; net absorption rate was reduced to 0.12 ml/h. This suggests the occurrence of the Na+/K+ pump. The marked reduction in the hydrothorax absorption produced by the inhibitors shows the occurrence of a solute-coupled liquid absorption from the pleural space. Liquid absorbed through the visceral pleura by the solute-coupled transport should be removed by the Starling forces of pulmonary capillaries. Solute-coupled absorption of liquid through the parietal pleura should oppose the filtration caused by the Starling forces.
通过在麻醉兔身上测量向右侧胸腔注入2ml林格溶液、含Na(+)-Cl-协同转运抑制剂的林格溶液或含Na+/K+泵抑制剂的林格溶液1小时后右侧胸腔内液体的体积，研究了溶质偶联的胸腔内液体吸收情况。注入林格溶液后收集的液体体积为1.56±0.08ml。初始体积为2.2ml，净吸收率为0.64ml/h。注入二磺基芪（0.1mM）或布美他尼（0.1mM）后收集的液体体积分别为2.01±0.06ml和2.01±0.05ml；净吸收率降至0.19ml/h。这表明存在Na(+)-Cl-协同转运。注入哇巴因（0.5mM）后收集的液体体积为2.08±0.08ml；净吸收率降至0.12ml/h。这表明存在Na+/K+泵。抑制剂导致胸腔积液吸收显著减少，表明存在溶质偶联的胸腔内液体吸收。通过溶质偶联转运经脏层胸膜吸收的液体应由肺毛细血管的Starling力清除。通过壁层胸膜的溶质偶联液体吸收应对抗由Starling力引起的滤过。
