Zocchi L, Agostoni E, Cremaschi D
Istituto di Fisiologia umana I, Università di Milano, Italy.
Respir Physiol. 1991 Oct;86(1):125-38. doi: 10.1016/0034-5687(91)90044-j.
The amounts of Na+ and Cl- in the right pleural space of anesthetized rabbits were determined 10 and 60 min after a 2 ml hydrothorax with the following solutions: Ringer, Ringer with an inhibitor of the Na(+)-Cl- coupled transport or of the Na+/K+ pump, Ringer with gluconate instead of Cl- or with methylglucamine instead of Na+. During the 10-60 min period: (a) with Ringer Na+ and Cl- decreased (P less than 0.01) along with an iso-osmotic liquid absorption, (b) with disulfonic-stilbene (0.1 mM), amiloride (0.7 mM), acetazolamide (0.1 mM), or ouabain (0.5 mM) Na+ did not change and Cl- decreased less (P less than 0.01) than with Ringer. With gluconate-Ringer or methylglucamine-Ringer the liquid flow reversed: in the former case Cl- and, to a smaller extent, Na+ increased (P less than 0.01); in the latter only Na+ increased (P less than 0.01). These findings suggest: (1) the occurrence of a Na+/H+ and Cl-/HCO3- double exchange on the serosal side and of a Na+/K+ pump on the interstitial side of the pleural mesothelium; (2) a slow efflux from the pleural space of gluconate or methylglucamine relative to the corresponding influx of Cl- or Na+, respectively; this drags liquid into the space by osmotic gradient.
在给麻醉的兔子胸腔内注入2 ml以下溶液后10分钟和60分钟，测定其右胸腔内Na⁺和Cl⁻的含量：林格液、含有Na⁺-Cl⁻协同转运抑制剂或Na⁺/K⁺泵抑制剂的林格液、用葡萄糖酸盐替代Cl⁻或用甲基葡糖胺替代Na⁺的林格液。在10 - 60分钟期间：(a) 注入林格液时，Na⁺和Cl⁻随着等渗液体的吸收而减少（P < 0.01）；(b) 注入二磺基芪（0.1 mM）、阿米洛利（0.7 mM）、乙酰唑胺（0.1 mM）或哇巴因（0.5 mM）时，Na⁺不变，Cl⁻减少的程度比注入林格液时小（P < 0.01）。用葡萄糖酸盐-林格液或甲基葡糖胺-林格液时，液体流动方向逆转：在前一种情况下，Cl⁻增加，Na⁺也有较小程度增加（P < 0.01）；在后一种情况下，只有Na⁺增加（P < 0.01）。这些发现表明：(1) 在胸膜间皮的浆膜侧发生Na⁺/H⁺和Cl⁻/HCO₃⁻双重交换，在间质侧存在Na⁺/K⁺泵；(2) 相对于相应的Cl⁻或Na⁺流入，葡萄糖酸盐或甲基葡糖胺从胸腔缓慢流出；这通过渗透梯度将液体吸入胸腔。
