工蜂大脑蛋白质组。
Worker honeybee brain proteome.
机构信息
Mass Spectrometry Group, Physics Department, CEADEN, Havana, Cuba.
出版信息
J Proteome Res. 2012 Mar 2;11(3):1485-93. doi: 10.1021/pr2007818. Epub 2012 Feb 1.
A large-scale mapping of the worker honeybee brain proteome was achieved by MudPIT. We identified 2742 proteins from forager and nurse honeybee brain samples; 17% of the total proteins were found to be differentially expressed by spectral count sampling statistics and a G-test. Sequences were compared with the EuKaryotic Orthologous Groups (KOG) catalog set using BLASTX and then categorized into the major KOG categories of most similar sequences. According to this categorization, nurse brain showed increased expression of proteins implicated in translation, ribosomal structure, and biogenesis (14.5%) compared with forager (1.8%). Experienced foragers overexpressed proteins involved in energy production and conversion, showing an extensive difference in this set of proteins (17%) in relation to the nurse subcaste (0.6%). Examples of proteins selectively expressed in each subcaste were analyzed. A comparison between these MudPIT experiments and previous 2-DE experiments revealed nine coincident proteins differentially expressed in both methodologies.
通过 MudPIT 实现了大规模的工蜂大脑蛋白质组图谱绘制。我们从采集蜂和看护蜂的大脑样本中鉴定出 2742 种蛋白质；通过光谱计数采样统计和 G 检验发现，其中 17%的总蛋白质表达水平存在差异。使用 BLASTX 将序列与真核同源群（KOG）目录集进行比较，然后根据最相似序列的主要 KOG 类别进行分类。根据这种分类，与采集蜂（1.8%）相比，看护蜂大脑中与翻译、核糖体结构和生物发生相关的蛋白质表达增加（14.5%）。有经验的采集蜂过度表达与能量产生和转化有关的蛋白质，与看护亚群（0.6%）相比，这一组蛋白质（17%）存在显著差异。分析了每个亚群中选择性表达的蛋白质。将这些 MudPIT 实验与之前的 2-DE 实验进行比较，发现两种方法均有 9 种差异表达的蛋白质重合。
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