Dairy Cattle Physiology Division, National Dairy Research Institute, Karnal, Haryana, India.
J Anim Physiol Anim Nutr (Berl). 2013 Apr;97(2):255-62. doi: 10.1111/j.1439-0396.2011.01264.x. Epub 2011 Dec 20.
This study was undertaken in buffalo neonates born to vitamin E (dl-alpha-tocopherol acetate)-supplemented and non-supplemented Murrah buffaloes. Calves from vitamin E-supplemented buffaloes (n = 10; vitamin E -supplemented calves [VeC]) and non-supplemented buffaloes (n = 10; control calves [CC]) constituted the treatment and control groups respectively. Two colostrum samples were taken at the first post-partum milking and again after 12 h from dams for IgG estimation. Sampling of blood was performed on days 0 (before colostrum feeding), 1, 3, 7, 14, 21, 28, 42, 56, 70, 84, 98, 112 and 126 post-birth and analysed for apparent efficiency of absorption (%) of IgG and various immune parameters. Colostral IgG level was significantly higher (p < 0.05) in vitamin E-supplemented buffaloes. The calves in both groups were born hypogammaglobulinemic with IgG level <5 g/l. However, first colostrum feeding resulted in significantly elevated IgG levels (>10 g/l) in calves of both groups at 24 h, which remained high afterwards. Apparent efficiency of absorption (%) of IgG at 24 h was significantly higher (p < 0.05) in VeC than in CC. Plasma Nitric Oxide (NO) levels were significantly elevated in the calves of either group at birth, which declined significantly (p < 0.01) afterwards. Vitamin E feeding to dams had no added effect on NO levels in experimental calves. Total leucocyte counts did not differ significantly between the two groups. However, lymphocyte and neutrophil counts changed significantly between groups (p < 0.01) and days (p < 0.01), with lymphocytes increasing and neutrophils declining with age. This study revealed that the calves were immunologically immature at birth. Ante-partum supplementation of vitamin E did not influence plasma NO or IgG but had a significant effect on colostral IgG (p < 0.05). It also improved the apparent efficiency of absorption (%) of IgG at 24 h in VeC as compared to CC.
本研究在维生素 E（dl-α-生育酚醋酸酯）补充和未补充的摩拉水牛所生的水牛新生儿中进行。来自维生素 E 补充水牛的小牛（n=10；维生素 E 补充小牛 [VeC]）和未补充水牛的小牛（n=10；对照小牛 [CC]）分别构成治疗组和对照组。在第一次产后挤奶时和产后 12 小时从母畜中再次采集两份初乳样本以进行 IgG 估计。在出生后第 0 天（初乳喂养前）、第 1 天、第 3 天、第 7 天、第 14 天、第 21 天、第 28 天、第 42 天、第 56 天、第 70 天、第 84 天、第 98 天、第 112 天和第 126 天进行采血，并分析 IgG 的表观吸收率（%）和各种免疫参数。补充维生素 E 的水牛的初乳 IgG 水平显著更高（p<0.05）。两组小牛出生时均为低丙种球蛋白血症，IgG 水平<5g/l。然而，首次初乳喂养使两组小牛在 24 小时内 IgG 水平显著升高（>10g/l），此后一直保持较高水平。24 小时时 VeC 的 IgG 表观吸收率（%）显著高于 CC（p<0.05）。两组小牛出生时血浆一氧化氮（NO）水平均显著升高，随后显著下降（p<0.01）。母体给与维生素 E 对实验组小牛的 NO 水平没有额外影响。两组之间的总白细胞计数没有显著差异。然而，淋巴细胞和中性粒细胞计数在组间（p<0.01）和天数（p<0.01）上有显著变化，随着年龄的增长，淋巴细胞增加，中性粒细胞减少。本研究表明，小牛出生时免疫尚未成熟。产前补充维生素 E 不会影响血浆 NO 或 IgG，但对初乳 IgG 有显著影响（p<0.05）。与 CC 相比，它还提高了 VeC 中 24 小时时 IgG 的表观吸收率（%）。
