Gao Ming-Qing, Hou Shou-Peng, Pu De-Qiang, Shi Min, Ye Gong-Yin, Chen Xue-Xin
Ministry of Agriculture Key Laboratory for Molecular Biology of Crop Insects and Pathogens, Institute of Insect Sciences, Zhejiang University, 268 Kaixuan Road, Hangzhou 310029, China.
Environ Entomol. 2010 Dec;39(6):2039-44. doi: 10.1603/EN10035.
Little is known about the potential cumulative long-term effects of transgenic crops on nontarget organisms. In the present laboratory study, the potential cumulative effects of transgenic Bacillus thuringiensis (Bt) rice on parasitoids in successive generations were observed for an egg parasitoid, Anagrus nilaparvatae parasitizing eggs of Nilaparvata lugens (Stål) (Hemiptera: Delphacidae) feeding on Bt rice. Enzyme-linked immunosorbent assay test confirmed that Cry1Ab insecticidal protein could be detected in newly eclosed parasitoid adults. However, no significant effect on the fecundity of Anagrus nilaparvatae Pang et Wang (Hymenoptera: Mymaridae) was observed between Bt and non-Bt rice. Developmental times of both genders of A. nilaparvatae parasitizing host eggs laid in Bt (KMD1 and KMD2) rice lines were significantly prolonged from first generation to second generation, but not always prolonged from third generation to 11th generation as compared with the control rice line. Furthermore, the sex ratio of A. nilaparvatae progeny from the first generation to 11th generation in three rice lines was not significantly different. In general, our results suggested that the effect of Bt rice on this parasitoid could be negligible.
关于转基因作物对非靶标生物潜在的长期累积影响，人们了解甚少。在本实验室研究中，以取食Bt水稻的褐飞虱（半翅目：飞虱科）的卵为寄主，观察了转Bt基因水稻对寄生蜂——稻虱缨小蜂连续世代的潜在累积影响。酶联免疫吸附试验证实，在新羽化的寄生蜂成虫中可检测到Cry1Ab杀虫蛋白。然而，Bt水稻和非Bt水稻之间，未观察到对稻虱缨小蜂繁殖力有显著影响。与对照水稻品系相比，寄生在Bt水稻品系（KMD1和KMD2）上的稻虱缨小蜂雌雄个体发育时间，从第一代到第二代显著延长，但从第三代到第十一代并不总是延长。此外，三个水稻品系中第一代到第十一代的稻虱缨小蜂后代的性别比例没有显著差异。总体而言，我们的结果表明，Bt水稻对这种寄生蜂的影响可以忽略不计。
