Akhtar Z R, Tian J C, Chen Y, Fang Q, Hu C, Chen M, Peng Y F, Ye G Y
State Key Laboratory of Rice Biology, Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310029, China.
Environ Entomol. 2010 Apr;39(2):715-26. doi: 10.1603/EN09095.
Nontarget impacts of six transgenic Bt rice lines (expressing the Cry1Ab or Cry1Ab/Cry1Ac protein) on the thrips, Stenchaetothrips biformis (Bagnall), attacking the rice seedling and tillering stages, were evaluated under laboratory and field conditions. Laboratory results showed relatively longer larval, pupal development and preoviposition durations of S. biformis. Although it had a shorter oviposition period, female adult longevity and less total laid eggs were found when fed on some tested Bt rice in comparison to non-Bt controls. S. biformis population dynamics in Bt and non-Bt plots were monitored using the plastic bag and beat plate methods. In the field, the temporal patterns of S. biformis population changes were similar between tested Bt rice lines and their respective control; however, the total number of S. biformis individuals collected from the Bt plots were significantly less or the same, varying from variety to variety, compared with those from the non-Bt plots. ELISA results showed that the Bt insecticidal protein could be transferred from Bt rice to the thrips, and the concentrations of the protein in rice leaves and thrips were not significantly correlated with some important biological parameters of the thrip. In addition, the potential effects of Bt rice on the abundance of S. biformis candidate predators are also discussed. In conclusion, our results show that the six Bt rice lines assessed may be less preferable host plants to S. biformis at the individual and population levels in comparison to the non-Bt rice plants.
在实验室和田间条件下，评估了6种转基因Bt水稻品系（表达Cry1Ab或Cry1Ab/Cry1Ac蛋白）对攻击水稻幼苗期和分蘖期的蓟马，即二点蓟马（Stenchaetothrips biformis (Bagnall)）的非靶标影响。实验室结果表明，二点蓟马的幼虫期、蛹期发育和产卵前期相对较长。虽然与非Bt对照相比，取食某些受试Bt水稻时其产卵期较短，但雌成虫寿命较短且总产卵量较少。使用塑料袋法和拍打法监测了Bt和非Bt地块中二点蓟马的种群动态。在田间，受试Bt水稻品系与其各自对照中二点蓟马种群变化的时间模式相似；然而，与非Bt地块相比，从Bt地块收集的二点蓟马个体总数显著较少或相同，因品种而异。酶联免疫吸附测定（ELISA）结果表明，Bt杀虫蛋白可从Bt水稻转移到蓟马体内，且水稻叶片和蓟马体内的蛋白浓度与蓟马的一些重要生物学参数无显著相关性。此外，还讨论了Bt水稻对二点蓟马候选捕食者数量的潜在影响。总之，我们的结果表明，与非Bt水稻植株相比，所评估的6种Bt水稻品系在个体和种群水平上对二点蓟马而言可能不是更适宜的寄主植物。
