Rostami Sima, Beech Robin Nicholas, Salavati Reza, Baneshi Mohammad Reza, Kamyabi Hossein, Harandi Majid Fasihi
Dept. of Parasitology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Institute of Parasitology, McGill University, Ste. Anne de Bellevue, Quebec, Canada.
Iran J Parasitol. 2013 Oct;8(4):579-85.
The purposes of the present study were morphometric characterization of rostellar hooks of Taenia multiceps and to investigate the association of hook length variation and the variability within two mitochondrial genes of sheep isolates of the parasite.
Up to 4500 sheep brains were examined for the presence of C. cerebralis. Biometric characters based on the larval rostellar hook size were measured for each individual isolate. Representative mitochondrial CO1 and 12S rRNA gene sequences for each of the isolates were obtained from NCBI GenBank. Morphometric and genetic data were analyzed using cluster analysis, Interclass Correlation Coefficient (ICC) and random effects model.
One hundred and fourteen sheep (2.5%) were found infected with the coenuri. The minimum and maximum number of scoleces per cyst was 40 and 550 respectively. Each scolex contained 22-27 hooks arranged in two rows of large and small hooks. The average total length of the large and small hooks was 158.9 and 112.1 μm, respectively. Using ICC, statistically significant clusters of different hook sizes were identified within the isolates. The length of the large and small hooks was significantly associated with the variability in mitochondrial 12S rRNA gene.
Taenia multiceps, is a relatively important zoonotic infection in Iranian sheep with the prevalence rate of 2.5%. Hook length analysis revealed statistically significant difference among individual isolates. Associations between the rostellar hook length and variability in the mitochondrial 12S rRNA was documented.
本研究的目的是对多头带绦虫头节小钩进行形态计量学特征分析，并研究该寄生虫绵羊分离株的小钩长度变异与两个线粒体基因变异之间的关联。
检查多达4500个绵羊脑，以确定是否存在脑多头蚴。对每个单独的分离株测量基于幼虫头节小钩大小的生物特征。从NCBI基因库获得每个分离株的代表性线粒体CO1和12S rRNA基因序列。使用聚类分析、组内相关系数（ICC）和随机效应模型分析形态计量学和遗传数据。
发现114只绵羊（2.5%）感染了共尾蚴。每个囊肿内头节的最小和最大数量分别为40和550个。每个头节包含22 - 27个小钩，排成两排大钩和小钩。大钩和小钩的平均总长度分别为158.9μm和112.1μm。使用ICC，在分离株中鉴定出不同小钩大小的具有统计学意义的聚类。大钩和小钩的长度与线粒体12S rRNA基因的变异显著相关。
多头带绦虫是伊朗绵羊中一种相对重要的人畜共患感染，患病率为2.5%。小钩长度分析显示各单独分离株之间存在统计学显著差异。记录了头节小钩长度与线粒体12S rRNA变异之间的关联。
