慢性鼻-鼻窦炎患者鼻窦组织中生物膜形成及 Toll 样受体 2、Toll 样受体 4 和 NF-κB 的表达。
Biofilm formation and Toll-like receptor 2, Toll-like receptor 4, and NF-kappaB expression in sinus tissues of patients with chronic rhinosinusitis.
机构信息
Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing Institute of Otolaryngology, Key Laboratory of Otolaryngology, Head and Neck Surgery, Ministry of Education, Beijing, China.
出版信息
Am J Rhinol Allergy. 2012 Mar-Apr;26(2):104-9. doi: 10.2500/ajra.2012.26.3718. Epub 2011 Dec 16.
BACKGROUND
Bacterial biofilm formation is associated with chronic rhinosinusitis (CRS). However, how bacterial biofilms are related to innate immune response in patients is poorly understood. The aim of this study was to evaluate the prevalence of biofilms in CRS patients and investigate the biofilm-related Toll-like receptor (TLR) and downstream NF-kappaB expression in sinus tissues of patients with CRS.
METHODS
A total of 31 CRS patients and 11 control patients with other diseases undergoing endoscopic sinus surgery were recruited consecutively. Their random sinonasal tissue specimens were examined for the formation of biofilms by scanning electron microscopy. The relative levels of TLR2, TLR4, and NF-kappaB expression in those sinus tissues were determined by quantitative real-time reverse-transcriptase polymerase chain reaction and immunohistochemistry.
RESULTS
Bacterial biofilms were detected in 20 of 31 samples from CRS patients but not in a single sample from control patients. The relative levels of TLR2, TLR4, and NF-kappaB expression in sinus tissues from CRS patients were significantly higher than that of controls, and the relative levels of TLR2 and NF-kappaB, but not TLR4, in biofilm-positive sinus tissues were significantly higher than that in biofilm-negative tissues of those CRS patients.
CONCLUSION
Our data indicated that bacterial biofilms were associated with higher levels of TLR2 and NF-kappaB in the majority of sinus tissues from patients with CRS.
背景
细菌生物膜的形成与慢性鼻-鼻窦炎（CRS）有关。然而，患者体内的细菌生物膜与先天免疫反应之间的关系尚不清楚。本研究旨在评估 CRS 患者生物膜的流行情况，并研究 CRS 患者鼻窦组织中与生物膜相关的 Toll 样受体（TLR）和下游 NF-κB 的表达。
方法
连续纳入 31 例 CRS 患者和 11 例患有其他疾病接受内镜鼻窦手术的对照患者。通过扫描电子显微镜检查他们随机的鼻-鼻窦组织标本中生物膜的形成。通过定量实时逆转录-聚合酶链反应和免疫组织化学测定这些鼻窦组织中 TLR2、TLR4 和 NF-κB 表达的相对水平。
结果
在 31 例 CRS 患者的 20 例样本中检测到细菌生物膜，但在对照组的 11 例样本中均未检测到。CRS 患者鼻窦组织中 TLR2、TLR4 和 NF-κB 的相对表达水平明显高于对照组，生物膜阳性鼻窦组织中 TLR2 和 NF-κB 的相对表达水平（但 TLR4 无差异）明显高于这些 CRS 患者生物膜阴性组织。
结论
我们的数据表明，在大多数 CRS 患者的鼻窦组织中，细菌生物膜与 TLR2 和 NF-κB 的水平升高有关。
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