辐射杂种图谱绘制：一种构建哺乳动物染色体高分辨率图谱的体细胞遗传学方法。

Radiation hybrid mapping: a somatic cell genetic method for constructing high-resolution maps of mammalian chromosomes.

作者信息

Cox D R, Burmeister M, Price E R, Kim S, Myers R M

机构信息

Department of Psychiatry, University of California, San Francisco 94143.

出版信息

Science. 1990 Oct 12;250(4978):245-50. doi: 10.1126/science.2218528.

DOI:10.1126/science.2218528

PMID:2218528

Abstract

Radiation hybrid (RH) mapping, a somatic cell genetic technique, was developed as a general approach for constructing long-range maps of mammalian chromosomes. This statistical method depends on x-ray breakage of chromosomes to determine the distances between DNA markers, as well as their order on the chromosome. In addition, the method allows the relative likelihoods of alternative marker orders to be determined. The RH procedure was used to map 14 DNA probes from a region of human chromosome 21 spanning 20 megabase pairs. The map was confirmed by pulsed-field gel electrophoretic analysis. The results demonstrate the effectiveness of RH mapping for constructing high-resolution, contiguous maps of mammalian chromosomes.

摘要

辐射杂种（RH）作图是一种体细胞遗传技术，它作为构建哺乳动物染色体长程图谱的通用方法而被开发出来。这种统计方法依靠染色体的X射线断裂来确定DNA标记之间的距离及其在染色体上的顺序。此外，该方法还能确定备选标记顺序的相对可能性。利用RH程序对来自人类21号染色体上一个跨度为20兆碱基对区域的14个DNA探针进行了作图。通过脉冲场凝胶电泳分析对该图谱进行了确认。结果证明了RH作图在构建哺乳动物染色体高分辨率连续图谱方面的有效性。