Walter M A, Spillett D J, Thomas P, Weissenbach J, Goodfellow P N
Department of Genetics, Cambridge University, UK.
Nat Genet. 1994 May;7(1):22-8. doi: 10.1038/ng0594-22.
In radiation hybrid mapping, chromosomes in human-rodent hybrid cells are fragmented by X-rays and fragments rescued by fusion of the donor cell to a recipient rodent cell. The co-retention frequencies of markers in 100-200 hybrids are used to map individual chromosomes, but mapping the whole genome in this way is impractical. We have reverted to the original protocols of Goss and Harris and have produced a panel of 44 hybrids using irradiated human fibroblasts as donors. This panel has been used to make a map of human chromosome 14 containing 40 ordered markers. The map integrates previously published maps and localizes nine new markers. We suggest that the construction of a high resolution map of the whole human genome is feasible with a single panel of 100-200 hybrids.
在辐射杂种细胞图谱绘制中，人-啮齿动物杂种细胞中的染色体通过X射线进行片段化处理，然后通过供体细胞与受体啮齿动物细胞融合来挽救片段。利用100 - 200个杂种细胞中标记物的共保留频率来绘制单个染色体图谱，但以这种方式绘制整个基因组图谱是不切实际的。我们已回归到戈斯和哈里斯最初的实验方案，并用经辐射的人成纤维细胞作为供体，构建了一组44个杂种细胞。该细胞组已用于绘制一张包含40个有序标记物的人类14号染色体图谱。该图谱整合了先前发表的图谱并定位了9个新标记物。我们认为，用一组100 - 200个杂种细胞构建一张高分辨率的整个人类基因组图谱是可行的。
