Islam Md Shihabul, Jahangir Chowdhury Arif, Rahi Md Sifat, Hasan Md Mahmudul, Sajib Salek Ahmed, Hoque Kazi Md Faisal, Reza Md Abu
Molecular Biology and Protein Science Laboratory, Department of Genetic Engineering and Biotechnology, Faculty of Life and Earth Science, University of Rajshahi, Rajshahi, 6205 Bangladesh.
Toxicol Res. 2019 Nov 21;36(1):79-88. doi: 10.1007/s43188-019-00011-7. eCollection 2020 Jan.
Cancer is the second death causing disease all over the world and until today 100 different types of cancer have been identified whose treatment methods consist of serious side effects on human body. To reduce the frequency of adverse effects of cancer treatment, nowadays plant derived natural components are getting priority. The plant is widely available in northern part of Bangladesh. The earlier researches suggested that popular varieties of some sp. like , etc. have good anti-proliferative activity. Hence, this study was designed to evaluate the anti-proliferative activity of leaf and bark extracts of against Ehrlich's ascites carcinoma (EAC) in vivo. The leaf and bark extracts of were used in several bioassays including Brine shrimp lethality test, hemagglutination activity test, antioxidant activity test, and cell growth inhibition test. Besides, fluorescence microscopy was performed to study apoptotic features in EAC cells, and molecular analysis like real-time PCR were also conducted. The results of Brine shrimp lethality test, hemagglutination activity test, and antioxidant activity assay supported the cell growth inhibition capability of leaf and bark extracts which was confirmed by in vivo cell growth inhibition bioassay. Moreover, the experimental extracts were able to induce cell apoptotis through altering the expression pattern of Bcl-2 and Bax genes. All of the results of this study suggest that several noble compounds are present in plant extracts which are capable for healing cancer cells.
癌症是全球第二大致死疾病，截至目前已识别出100种不同类型的癌症，其治疗方法会对人体产生严重副作用。为降低癌症治疗不良反应的发生率，如今植物来源的天然成分备受关注。这种植物在孟加拉国北部广泛分布。早期研究表明，某些品种如 、 等具有良好的抗增殖活性。因此，本研究旨在评估该植物叶和树皮提取物对艾氏腹水癌（EAC）的体内抗增殖活性。该植物的叶和树皮提取物用于多种生物测定，包括卤虫致死试验、血凝活性试验、抗氧化活性试验和细胞生长抑制试验。此外，通过荧光显微镜研究EAC细胞的凋亡特征，并进行实时PCR等分子分析。卤虫致死试验、血凝活性试验和抗氧化活性测定的结果支持了叶和树皮提取物的细胞生长抑制能力，这在体内细胞生长抑制生物测定中得到了证实。此外，实验提取物能够通过改变Bcl-2和Bax基因的表达模式诱导细胞凋亡。本研究的所有结果表明，该植物提取物中存在几种有价值的化合物，它们能够治愈癌细胞。
