August Kirchenstein Institute of Microbiology and Virology, Riga Stradins University, Latvia.
Medicina (Kaunas). 2011;47(10):527-31.
Recent studies have focused on the associations between human herpesvirus 6 (HHV-6) and human herpesvirus 7 (HHV-7), and multiple sclerosis (MS). The aim of this study was to investigate the associations between HHV-6 and HHV-7 reactivation and MS disease activity, and interleukin 12 (IL-12) and tumor necrosis factor α (TNF-α) production.
The frequency of plasma viremia by nested polymerase chain reaction and transcription of viral mRNA in peripheral blood mononuclear cells by reverse transcriptase-polymerase chain reaction (RT-PCR) of 14 relapsing/remitting (RR) and 14 secondary progressive (SP) MS patients were studied in comparison with clinical manifestation of the disease. Serum concentrations of cytokines IL-12 and TNF-α were analyzed by enzyme-linked immunosorbent assay.
Plasma samples from 25 of the 28 MS patients with estimated latent/persistent HHV-6 and/or HHV-7 infection were examined during relapse and remission/relative remission. HHV-6 reactivation was found in 4 of the 7 RRMS and 4 of the 7 SPMS patients, and HHV-7 reactivation was identified in 3 of the 7 RRMS and 1 of the 7 SPMS patients (all in relapse). In 2 of the 3 RRMS patients without viremia in relapse, HHV-6 mRNA transcription was detected. In RRMS and SPMS patients with active HHV-6 and HHV-7 infection in relapse, the serum concentrations of IL-12 and TNF-α were significantly higher than in those with latent virus infection.
HHV-6 and HHV-7 reactivation could be implicated in the exacerbation of MS via activation of Th1 lymphocyte subsets.
研究人类疱疹病毒 6（HHV-6）和人类疱疹病毒 7（HHV-7）与多发性硬化（MS）之间的关联，以及白细胞介素 12（IL-12）和肿瘤坏死因子α（TNF-α）的产生。
通过巢式聚合酶链反应检测 14 例复发缓解型（RR）和 14 例继发进展型（SP）MS 患者的血浆病毒血症频率，并通过逆转录聚合酶链反应（RT-PCR）检测外周血单个核细胞中病毒 mRNA 的转录，同时与疾病的临床表现进行比较。采用酶联免疫吸附试验分析细胞因子 IL-12 和 TNF-α的血清浓度。
在 28 例 MS 患者中，有 25 例患者估计存在潜伏/持续性 HHV-6 和/或 HHV-7 感染，在复发和缓解/相对缓解期间检测了其血浆样本。在 7 例 RRMS 和 7 例 SPMS 患者中发现了 HHV-6 再激活，在 7 例 RRMS 和 1 例 SPMS 患者中发现了 HHV-7 再激活（均在复发时）。在 3 例 RRMS 患者中，有 2 例在复发时无病毒血症，但检测到 HHV-6 mRNA 转录。在 RRMS 和 SPMS 患者中，活跃的 HHV-6 和 HHV-7 感染与潜伏病毒感染相比，血清中 IL-12 和 TNF-α的浓度明显更高。
HHV-6 和 HHV-7 的再激活可能通过激活 Th1 淋巴细胞亚群而导致 MS 恶化。
