Department of Gastroenterology, Peking Union Medical College Hospital, Chinese Academy of Medical Science, Beijing, China.
J Dig Dis. 2012 Jan;13(1):24-32. doi: 10.1111/j.1751-2980.2011.00551.x.
Colon cancer is more common in the USA and Europe than that in China, for reasons that are unclear. The aim of this study was to investigate the differences in gene expression profiles and carcinogenesis pathways between colon and rectal cancer.
Expression profiling of primary tumor tissues from 12 colon and 12 rectal cancers was performed using oligonucleotide microarray analysis. All samples were strictly matched by clinical features. Bioinformatic analyses such as the Kyoto Encyclopedia of Genes and Genomes were used to identify genes and pathways specifically associated with colon or rectal cancers.
A total of 824 genes were differentially expressed in colon and rectal cancers. All differential gene interactions in the Signal-Net were analyzed. More genes were differentially expressed and included in the Signal-Net for rectal than colon cancer. Of the genes differentially expressed between colon and rectal cancer, S100P, the Reg family, ACTN1, CAMK2G and ACAT1 were the most significantly altered. Genes involved in the cell cycle pathway were present in rectal and colon cancers, but were more important in rectal cancer. The p53 and metabolic signaling pathways were significantly different in colon and rectal cancers. Gene expression profiles differed between colon and rectal cancer, with metabolic pathways being more important in rectal cancer.
The oncogenesis of rectal cancer may be more complex than that of colon cancer. Some genes could be new biomarkers for distinguishing between these two cancers.
结肠癌在美国和欧洲比在中国更为常见，但原因尚不清楚。本研究旨在探讨结肠癌和直肠癌之间基因表达谱和致癌途径的差异。
采用寡核苷酸微阵列分析对 12 例结肠癌和 12 例直肠癌的原发肿瘤组织进行表达谱分析。所有样本均通过临床特征严格匹配。京都基因与基因组百科全书等生物信息学分析用于鉴定与结肠癌或直肠癌特异性相关的基因和途径。
结肠癌和直肠癌中共有 824 个基因差异表达。对信号网络中的所有差异基因相互作用进行了分析。直肠癌比结肠癌有更多差异表达的基因被纳入信号网络。在结肠癌和直肠癌之间差异表达的基因中，S100P、Reg 家族、ACTN1、CAMK2G 和 ACAT1 改变最为显著。细胞周期途径中涉及的基因存在于直肠癌和结肠癌中，但在直肠癌中更为重要。p53 和代谢信号通路在结肠癌和直肠癌中存在显著差异。结肠癌和直肠癌的基因表达谱不同，代谢途径在直肠癌中更为重要。
直肠癌的致癌机制可能比结肠癌更为复杂。一些基因可能是区分这两种癌症的新生物标志物。
