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基于化学发光的生物传感器用于定量检测玉米样品中的伏马菌素。

Chemiluminescence-based biosensor for fumonisins quantitative detection in maize samples.

机构信息

Department of Pharmaceutical Sciences, Alma Mater Studiorum - University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy.

出版信息

Biosens Bioelectron. 2012 Feb 15;32(1):283-7. doi: 10.1016/j.bios.2011.11.039. Epub 2011 Dec 2.

Abstract

A compact portable chemiluminescent biosensor for simple, rapid, and ultrasensitive on-site quantification of fumonisins (fumonisin B1+fumonisin B2) in maize has been developed. The biosensor integrates a competitive lateral flow immunoassay based on enzyme-catalyzed chemiluminescence detection and a highly sensitive portable charge-coupled device (CCD) camera, employed in a contact imaging configuration. The use of chemiluminescence detection allowed accurate and objective analyte quantification, rather than qualitative or semi-quantitative information usually obtained employing conventional lateral flow immunoassays based on colloidal gold labeling. A limit of detection of 2.5 μgL(-1) for fumonisins was achieved, with an analytical working range of 2.5-500 μgL(-1) (corresponding to 25-5000 μgkg(-1) in maize flour samples, according to the extraction procedure). Total assay time was 25 min, including sample preparation. A simple and convenient extraction procedure, performed by suspending the sample in a buffered solution and rapidly heating to eliminate endogenous peroxidase enzyme activity was employed for maize flour samples analysis, obtaining recoveries in the range 90-115%, when compared with LC-MS/MS analysis. The chemiluminescence immunochromatography-based biosensor is a rapid, low cost portable test suitable for point-of-use applications.

摘要

一种紧凑便携的化学发光生物传感器,用于简单、快速、超灵敏地现场定量玉米中的伏马菌素(伏马菌素 B1+伏马菌素 B2)。该生物传感器集成了基于酶催化化学发光检测的竞争性横向流动免疫分析和高灵敏度便携式电荷耦合器件(CCD)相机,采用接触成像配置。化学发光检测的使用允许对分析物进行准确和客观的定量,而不是通常使用基于胶体金标记的传统横向流动免疫分析获得的定性或半定量信息。伏马菌素的检测限达到 2.5 μgL(-1),分析工作范围为 2.5-500 μgL(-1)(根据提取程序,相当于玉米粉样品中的 25-5000 μgkg(-1))。总分析时间为 25 分钟,包括样品制备。对于玉米粉样品分析,采用了一种简单方便的提取程序,将样品悬浮在缓冲溶液中并快速加热以消除内源性过氧化物酶的活性,与 LC-MS/MS 分析相比,回收率在 90-115%范围内。基于化学发光免疫层析的生物传感器是一种快速、低成本的便携式测试方法,适用于现场应用。

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