Brain and Cognitive Sciences, College of Natural Sciences, Seoul National University, Seoul 151-747, Korea.
BMB Rep. 2011 Dec;44(12):793-8. doi: 10.5483/bmbrep.2011.44.12.793.
Recently, pluripotency induction or cellular reprogramming by introducing critical transcription factors has been extensively studied, but has been demonstrated only in vitro. Based on reports that Oct4 is critically involved in transforming neural stem cells into pluripotent cells, we used the lentiviral vector to introduce the Oct4 gene into the hippocampal dentate gyrus (DG) of adult mice. We examined whether this manipulation led to cellular or behavioral changes, possibly through processes involving the transformation of NS cells into pluripotent cells. The Oct4 lentivirus-infused group and the green fluorescent protein lentivirus-infused group showed a similar thickness of the DG and a comparable level of synaptophysin expression in the DG. Furthermore, our behavioral analyses did not show any differences between the groups concerning exploratory activity, anxiety, or memory abilities. This first trial for pluripotency induction in vivo, despite negative results, provides implications and information for future studies on in vivo cellular reprogramming.
最近，通过引入关键转录因子进行多能性诱导或细胞重编程已经得到了广泛研究，但仅在体外得到了证实。基于 Oct4 蛋白在将神经干细胞转化为多能细胞过程中起着关键作用的报道，我们使用慢病毒载体将 Oct4 基因导入成年小鼠的海马齿状回（DG）。我们研究了这种操作是否会导致细胞或行为的变化，可能是通过将 NS 细胞转化为多能细胞的过程。Oct4 慢病毒感染组和绿色荧光蛋白慢病毒感染组的 DG 厚度相似，DG 中的突触素表达水平也相当。此外，我们的行为分析没有显示出两组之间在探索性活动、焦虑或记忆能力方面有任何差异。尽管结果为阴性，但这是首次在体内进行多能性诱导的尝试，为未来的体内细胞重编程研究提供了启示和信息。
