Department of Medical Microbiology and Parasitology, University of Lagos, Lagos, Nigeria.
Clin Microbiol Infect. 2012 Mar;18(3):E49-51. doi: 10.1111/j.1469-0691.2011.03730.x. Epub 2011 Dec 22.
Of 109 clinical Escherichia coli isolates from two major tertiary hospitals in Lagos (University Teaching Hospital and the National Orthopaedic Hospital Igbobi), 14 (12.8%) extended-spectrum beta-lactamse (ESBL) producers were characterized using PCR and sequencing, ERIC-PCR and multilocus sequence typing. All ESBL-producing isolates encoded only the CTX-M-15 gene. Clonal group ST131 (35.7%) was the predominant ST, followed by ST617 (28.6%). Isolated cases of other sequence types were also observed. Plasmid-mediated quinolone resistance genes qnrA, qnrB1 and aac-(6')-lb-cr were detected among these ESBL isolates of different clonal groups. This is the first description of the clonality of CTX-M-15-producing E. coli from Nigeria. The presence of diverse clonal lineages shows the continuing potential for genetic diversification and emergence of new epidemic strains.
从拉各斯的两家主要的三级医院（大学教学医院和国家骨科医院伊格博比）的 109 株临床大肠杆菌分离株中，通过 PCR 和测序、ERIC-PCR 和多位点序列分型，对 14 株（12.8%）产超广谱β-内酰胺酶（ESBL）的分离株进行了特征分析。所有产 ESBL 的分离株仅编码 CTX-M-15 基因。克隆群 ST131（35.7%）是主要的 ST，其次是 ST617（28.6%）。还观察到其他序列类型的孤立病例。在这些不同克隆群的 ESBL 分离株中检测到了质粒介导的喹诺酮耐药基因 qnrA、qnrB1 和 aac-(6')-lb-cr。这是首次描述尼日利亚产 CTX-M-15 的大肠杆菌的克隆性。不同克隆谱系的存在表明了遗传多样化和新的流行株出现的持续潜力。
