Department of Microbiology and Immunology, University of Gothenburg, P.O. Box 435, S-40530 Gothenburg, Sweden.
Microbiol Res. 2012 Oct 12;167(9):564-70. doi: 10.1016/j.micres.2011.11.006. Epub 2011 Dec 20.
Establishment of a simple and rapid multiplex PCR system for identification of the main diarrheagenic E. coli categories, including enteroaggregative E. coli, enterotoxigenic E. coli, enteropathogenic E. coli, and enterohemorrhagic E. coli, is described. This two-step multiplex PCR system allows the identification by targeting CVD432, LT, STh, STp, Eae, Bfp, Stx1, and Stx2. By applying the developed multiplex PCR system, categorization of E. coli isolates isolated from stool samples of infants with diarrhea into the main diarrheagenic E. coli categories is also shown.
本文描述了一种用于鉴定主要致泻性大肠杆菌（包括聚集性肠致病性大肠杆菌、肠毒素性大肠杆菌、肠致病性大肠杆菌和肠出血性大肠杆菌）的简单快速多重 PCR 系统。该两步多重 PCR 系统通过靶向 CVD432、LT、STh、STp、Eae、Bfp、Stx1 和 Stx2 来进行鉴定。通过应用所开发的多重 PCR 系统，还将从腹泻婴儿粪便样本中分离出的大肠杆菌菌株分类为主要致泻性大肠杆菌。
