Genome Assessment of Carbapenem- and Colistin-Resistant from Patients in a Sentinel Hospital in China.

Antimicrobial-resistant (AMR) pathogens are a significant threat to public health worldwide. However, the primary carrier of AMR genes, particularly against last-resort antibiotics, is still only partially studied in Chinese hospitals. In a sentinel hospital in China, we collected 157 strains from patients between January and July 2021. One -, nine -, and one -positive recovered from inpatients were identified as resistant to meropenem and colistin. There are 37 virulence genes discovered in the 11 strains, including in strain EC21Z-147 (O128: H4), which belongs to the enteroaggregative (EAEC). The gene is distributed into distinct ST types, including ST48, ST616, ST410, ST711, and ST2003, while the gene was identified in ST117. The conjugative plasmids IncX3, IncI1-I, and IncI2 mediated the and genes detected among inpatients. Notably, the youngest age at which -positive has been reported was at one day old, in a child in which the strain is closely related to strains with animal origins. Hospitals are major environments for the spread and dissemination of critical virulence and AMR genes, which requires active monitoring systems at the genome level to surveil the spread of virulence and AMR.