用于同时鉴定与肠道感染相关的六类致腹泻性大肠杆菌的单一多重聚合酶链反应检测法。
Single multiplex PCR assay to identify simultaneously the six categories of diarrheagenic Escherichia coli associated with enteric infections.
作者信息
Vidal Maricel, Kruger Eileen, Durán Claudia, Lagos Rosanna, Levine Myron, Prado Valeria, Toro Cecilia, Vidal Roberto
机构信息
Programa de Microbiología, ICBM, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
出版信息
J Clin Microbiol. 2005 Oct;43(10):5362-5. doi: 10.1128/JCM.43.10.5362-5365.2005.
Abstract
We designed a multiplex PCR for the detection of all categories of diarrheagenic Escherichia coli. This method proved to be specific and rapid in detecting virulence genes from Shiga toxin-producing (stx(1), stx(2), and eae), enteropathogenic (eae and bfp), enterotoxigenic (st II and lt), enteroinvasive (vir F and ipa H), entero-aggregative (aaf II), and diffuse adherent (daa E) Escherichia coli in stool samples.
摘要
我们设计了一种多重聚合酶链反应（multiplex PCR）用于检测各类致泻性大肠杆菌。该方法在检测粪便样本中产生志贺毒素的大肠杆菌（stx(1)、stx(2) 和 eae）、肠致病性大肠杆菌（eae 和 bfp）、肠产毒性大肠杆菌（st II 和 lt）、肠侵袭性大肠杆菌（vir F 和 ipa H）、肠聚集性大肠杆菌（aaf II）以及弥漫性黏附性大肠杆菌（daa E）的毒力基因时，被证明具有特异性且快速。
相似文献
1
Single multiplex PCR assay to identify simultaneously the six categories of diarrheagenic Escherichia coli associated with enteric infections.用于同时鉴定与肠道感染相关的六类致腹泻性大肠杆菌的单一多重聚合酶链反应检测法。
J Clin Microbiol. 2005 Oct;43(10):5362-5. doi: 10.1128/JCM.43.10.5362-5365.2005.
2
Multiplex PCR assay for identification of human diarrheagenic Escherichia coli.用于鉴定人致腹泻性大肠杆菌的多重聚合酶链反应检测法
J Clin Microbiol. 2003 Jun;41(6):2669-71. doi: 10.1128/JCM.41.6.2669-2671.2003.
3
4
Shiga toxin-negative attaching and effacing Escherichia coli: distinct clinical associations with bacterial phylogeny and virulence traits and inferred in-host pathogen evolution.志贺毒素阴性紧密黏附并损伤肠上皮的大肠杆菌：与细菌系统发育和毒力特征的不同临床关联以及推断的宿主体内病原体进化
Clin Infect Dis. 2008 Jul 15;47(2):208-17. doi: 10.1086/589245.
5
Simple and rapid multiplex PCR for identification of the main human diarrheagenic Escherichia coli.用于鉴定主要致人类腹泻大肠杆菌的简单快速多重 PCR。
Microbiol Res. 2012 Oct 12;167(9):564-70. doi: 10.1016/j.micres.2011.11.006. Epub 2011 Dec 20.
6
7
Octaplex PCR and fluorescence-based capillary electrophoresis for identification of human diarrheagenic Escherichia coli and Shigella spp.用于鉴定人致泻性大肠杆菌和志贺氏菌属的八重PCR及荧光毛细管电泳法
J Microbiol Methods. 2007 Feb;68(2):331-41. doi: 10.1016/j.mimet.2006.09.013. Epub 2006 Oct 31.
8
Single multiplex polymerase chain reaction to detect diverse loci associated with diarrheagenic Escherichia coli.用于检测与致泻性大肠杆菌相关的不同基因座的单一多重聚合酶链反应
Emerg Infect Dis. 2003 Jan;9(1):127-31. doi: 10.3201/eid0901.010507.
9
Detection of the cytolethal distending toxin locus cdtB among diarrheagenic Escherichia coli isolates from humans in Iran.伊朗人类腹泻性大肠杆菌分离株中细胞致死性膨胀毒素基因座cdtB的检测
Res Microbiol. 2005 Mar;156(2):137-44. doi: 10.1016/j.resmic.2004.09.011. Epub 2004 Dec 1.
10
Rapid detection of diarrheagenic E. coli by real-time PCR.通过实时聚合酶链反应快速检测致泻性大肠杆菌
J Microbiol Methods. 2005 Jun;61(3):335-41. doi: 10.1016/j.mimet.2004.12.007. Epub 2005 Jan 11.
引用本文的文献
1
Could Exotic Birds Play a Significant Role in the Emergence of Antibiotic-Resistant Microorganisms?外来鸟类会在抗生素耐药微生物的出现中扮演重要角色吗？
Curr Microbiol. 2025 Feb 28;82(4):161. doi: 10.1007/s00284-025-04134-z.
2
An outbreak of acute diarrhoeal disease caused by Shigella sonnei in a village in Dibrugarh district, Assam.在阿萨姆邦迪布鲁加尔区的一个村庄发生了由宋内志贺菌引起的急性腹泻病暴发。
Indian J Med Res. 2024 Jul;160(1):87-94. doi: 10.25259/ijmr_1949_23.
3
Bacteriophage defends murine gut from Escherichia coli invasion via mucosal adherence.噬菌体通过黏膜黏附保护小鼠肠道免受大肠杆菌侵袭。
Nat Commun. 2024 Jun 4;15(1):4764. doi: 10.1038/s41467-024-48560-2.
4
Characteristics of Pathogenic Associated with Diarrhea in Children under Five Years in Northwestern Ethiopia.埃塞俄比亚西北部五岁以下儿童腹泻相关病原菌的特征
Trop Med Infect Dis. 2024 Mar 21;9(3):65. doi: 10.3390/tropicalmed9030065.
5
Multiple-Drug Resistant Shiga Toxin-Producing in Raw Milk of Dairy Bovine.奶牛生乳中多重耐药性产志贺毒素菌
Trop Med Infect Dis. 2024 Mar 19;9(3):64. doi: 10.3390/tropicalmed9030064.
6
Thermal Adaptation Alters Response to Thermal Stress and Expression of Virulent Genes (eae, stx1, stx2, and hlyA) in Pathogenic Escherichia coli Isolated from Pork.热适应改变了从猪肉中分离的致病性大肠杆菌对热应激的反应和毒力基因（eae、stx1、stx2 和 hlyA）的表达。
Curr Microbiol. 2023 Aug 26;80(10):330. doi: 10.1007/s00284-023-03446-2.
7
Are non-lactose-fermenting important diarrhoeal pathogens in children and adults?非乳糖发酵菌是儿童和成人重要的腹泻病原体吗？
Access Microbiol. 2023 Jul 12;5(7). doi: 10.1099/acmi.0.000459.v3. eCollection 2023.
8
PCR diagnostics are insufficient for the detection of Diarrhoeagenic Escherichia coli in Ibadan, Nigeria.聚合酶链反应诊断方法不足以检测尼日利亚伊巴丹的致泻性大肠杆菌。
PLOS Glob Public Health. 2023 Aug 7;3(8):e0001539. doi: 10.1371/journal.pgph.0001539. eCollection 2023.
9
Phylodynamics Uncovers the Transmission of Antibiotic-Resistant between Canines and Humans in an Urban Environment.系统发育动力学揭示了城市环境中犬类与人类之间抗生素抗性的传播。
bioRxiv. 2023 Jun 1:2023.06.01.543064. doi: 10.1101/2023.06.01.543064.
10
Detection and analysis of Shiga toxin producing and enteropathogenic Escherichia coli in cattle from Tierra del Fuego, Argentina.阿根廷火地岛牛源产志贺毒素和肠致病性大肠杆菌的检测与分析。
Braz J Microbiol. 2023 Jun;54(2):1257-1266. doi: 10.1007/s42770-023-00958-8. Epub 2023 Apr 11.
本文引用的文献
1
Multiplex PCR for diagnosis of enteric infections associated with diarrheagenic Escherichia coli.用于诊断与致泻性大肠杆菌相关的肠道感染的多重聚合酶链反应
J Clin Microbiol. 2004 Apr;42(4):1787-9. doi: 10.1128/JCM.42.4.1787-1789.2004.
2
Typical and atypical enteropathogenic Escherichia coli.典型和非典型肠道致病性大肠杆菌
Emerg Infect Dis. 2002 May;8(5):508-13. doi: 10.3201/eid0805.010385.
3
Expression of the virulence plasmid-carried apyrase gene (apy) of enteroinvasive Escherichia coli and Shigella flexneri is under the control of H-NS and the VirF and VirB regulatory cascade.侵袭性大肠杆菌和福氏志贺氏菌携带的毒力质粒上的腺苷三磷酸双磷酸酶基因（apy）的表达受H-NS以及VirF和VirB调控级联的控制。
Infect Immun. 1998 Oct;66(10):4957-64. doi: 10.1128/IAI.66.10.4957-4964.1998.
4
Virulence of enterohemorrhagic Escherichia coli: role of molecular crosstalk.肠出血性大肠杆菌的毒力：分子串扰的作用
Trends Microbiol. 1998 Jun;6(6):228-33. doi: 10.1016/s0966-842x(98)01282-7.
5
Enteroaggregative Escherichia coli.肠集聚性大肠杆菌
Emerg Infect Dis. 1998 Apr-Jun;4(2):251-61. doi: 10.3201/eid0402.980212.
6
7
Diarrheagenic Escherichia coli.致泻性大肠杆菌
Clin Microbiol Rev. 1998 Jan;11(1):142-201. doi: 10.1128/CMR.11.1.142.
8
Aggregative adherence fimbria II, a second fimbrial antigen mediating aggregative adherence in enteroaggregative Escherichia coli.凝聚性黏附菌毛II，介导肠集聚性大肠埃希菌凝聚性黏附的第二种菌毛抗原。
Infect Immun. 1997 Oct;65(10):4135-45. doi: 10.1128/iai.65.10.4135-4145.1997.
9
Characterization of HEp-2 cell projection formation induced by diffusely adherent Escherichia coli.弥漫性黏附大肠杆菌诱导的HEp-2细胞突起形成的特征分析
Microb Pathog. 1996 Dec;21(6):421-34. doi: 10.1006/mpat.1996.0073.
10
Secretion of extracellular proteins by enterohemorrhagic Escherichia coli via a putative type III secretion system.肠出血性大肠杆菌通过一种假定的III型分泌系统分泌细胞外蛋白。
Infect Immun. 1996 Nov;64(11):4826-9. doi: 10.1128/iai.64.11.4826-4829.1996.
Zotero 插件