Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, PR China.
Analyst. 2012 Feb 21;137(4):1020-3. doi: 10.1039/c2an15962g. Epub 2011 Dec 23.
In this work, by incorporating a specific DNAzyme sequence into a hairpin aptamer probe, we describe a label-free and sensitive method for electrochemical detection of cytokines using recombinant human IFN-γ as the model analyte. The hairpin aptamer probes are immobilized on a gold electrode through self-assembly. The presence of IFN-γ opens the hairpin structure and forms the hemin/G-quadruplex peroxidase-mimicking DNAzyme with subsequent addition of hemin. The peroxidase-mimicking DNAzyme catalyzes the electro-reduction of H(2)O(2) and amplifies the current response for IFN-γ detection, which enables the monitoring of IFN-γ at the sub-nanomolar level. The proposed sensor also shows high selectivity towards the target analyte. Our strategy thus opens new opportunities for label-free and amplified detection of different types of cytokines.
在这项工作中，我们通过将特定的 DNA 酶序列整合到发夹适体探针中，描述了一种无需标记且灵敏的电化学检测细胞因子的方法，使用重组人 IFN-γ 作为模型分析物。发夹适体探针通过自组装固定在金电极上。IFN-γ 的存在会打开发夹结构，并与随后加入的血红素形成血红素/G-四链体过氧化物酶模拟 DNA 酶。过氧化物酶模拟 DNA 酶催化 H(2)O(2)的电还原，并放大 IFN-γ 检测的电流响应，从而能够在亚纳摩尔水平监测 IFN-γ。该传感器还对目标分析物表现出很高的选择性。因此，我们的策略为无需标记和放大检测不同类型的细胞因子开辟了新的机会。
