State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
Biosens Bioelectron. 2012 Feb 15;32(1):208-12. doi: 10.1016/j.bios.2011.12.011. Epub 2011 Dec 13.
We report an aptasensor for biosensing of Ochratoxin A (OTA) using aptamer-DNAzyme hairpin as biorecognition element. The structure of this engineered nucleic acid includes the horseradish peroxidase (HRP)-mimicking DNAzyme and the OTA specific aptamer sequences. A blocking tail captures a part of these sequences in the stem region of the hairpin. In the presence of OTA, the hairpin is opened due to the formation of the aptamer-analyte complex. As a result, self-assembly of the active HRP-mimicking DNAzyme occurs. The activity of this DNAzyme is linearly correlated with OTA concentration up to 10 nM, showing a limit of detection of 2.5 nM.
我们报道了一种基于适体-DNA zyme 发夹作为生物识别元件的适体传感器，用于生物传感赭曲霉毒素 A（OTA）。这种工程化核酸的结构包括辣根过氧化物酶（HRP）模拟 DNAzyme 和 OTA 特异性适体序列。一个阻断尾在发夹的茎区捕获这些序列的一部分。在 OTA 的存在下，由于适体-分析物复合物的形成，发夹被打开。结果，具有活性的 HRP 模拟 DNAzyme 发生自组装。该 DNAzyme 的活性与 OTA 浓度呈线性相关，直到 10 nM，检测限为 2.5 nM。
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