Senturk Gozde Erkanli, Isler Cihan, Kemerdere Rahsan, Ulu Mustafa Onur, Umay Cenk, Ozkaymakci Aydan, Aker Gulhan Rezzan, Ercan Feriha, Ozkara Cigdem, Onat Filiz, Uzan Mustafa
Marmara University, Faculty of Medicine, Department of Histoogy and Embryology, Istanbul, Turkey.
Turk Neurosurg. 2011;21(4):454-60.
In utero irradiation models induce diffuse neuronal damage. Experimental studies have shown that hyperthermia induced seizures are easily elicited and have high mortality accompanied by neuronal loss. Neuronal damage and loss are the results of cell death coupled with cortical development in altered cellular development. The aim of the study was to investigate the changes in hippocampus that was exposed to irradiation and hyperthermia.
Four groups were studied: 1) The irradiation group was exposed to 225 cGy irradiation on the 17th gestational day; 2) The hyperthermia group was exposed to hyperthermia on the 10th postnatal day; 3) The hyperthermia plus irradiation group was exposed to in utero irradiation and postnatal hyperthermia; 4) The control group was sham operated. Animals were examined 3 and 6 months later.
The hippocampus was atrophic with neuronal loss in CA regions and ectopic neurons were in irradiation group. Severe damage with the most atrophy was demonstrated in all regions of the irradiation plus hyperthermia group. In long term, damage was severe in all groups.
This study demonstrated more damage in hippocampi exposed to both irradiation and hyperthermia that may be taken as an evidence for the double hit hypothesis in the development of hippocampal damage.
子宫内照射模型可导致弥漫性神经元损伤。实验研究表明，热诱导的癫痫发作很容易引发，且死亡率高，伴有神经元丢失。神经元损伤和丢失是细胞死亡与细胞发育改变时皮质发育相结合的结果。本研究的目的是调查暴露于照射和热环境下的海马体的变化。
研究分为四组：1）照射组在妊娠第17天接受225 cGy照射；2）热疗组在出生后第10天接受热疗；3）热疗加照射组接受子宫内照射和出生后热疗；4）对照组进行假手术。在3个月和6个月后对动物进行检查。
照射组海马体萎缩，CA区有神经元丢失，且存在异位神经元。照射加高温组所有区域均显示出严重损伤和最明显的萎缩。长期来看，所有组的损伤都很严重。
本研究表明，暴露于照射和热环境下的海马体损伤更严重，这可能是海马体损伤发展中双重打击假说的一个证据。
