Minasi L B, Costa E O A, Silva D M, Melo C O A, de Almeida J G, Vieira T C, Silva Júnior R L, Ribeiro C L, da Silva C C, da Cruz A D
Programa de Pós-Graduação Stricto Sensu, Doutorado em Biologia Celular e Molecular, Universidade Federal de Goiás, Goiânia, GO, Brasil.
Genet Mol Res. 2011 Dec 12;10(4):3924-9. doi: 10.4238/2011.December.12.5.
The frequency of micronuclei in both buccal cells and peripheral blood lymphocytes is extensively used as a biomarker of chromosomal damage and genome stability in human populations. We examined whether prolonged exposure to complex mixtures of pesticides leads to an increase in cytogenetic damage. The exposed group comprised 50 agricultural aviators, mainly from Central and Southeast regions of Brazil, who had inhaled agrochemicals for more than 10 years without personal protection equipment; the control group consisted of 17 men from the same regions, without indication of exposure to pesticides, There were three times higher frequencies of micronuclei (P < 0.05) and 2.5 times higher frequencies of binucleated cells in the aviators when compared to controls. However, cytotoxic alterations such as broken eggs and karyorrhexis did not present statistically significant differences between the exposed and control groups. Therefore, diverse agrochemicals used to combat pests in agriculture possess genotoxic effects in the oral mucosa of the agricultural pilots, as showed in this study.
口腔细胞和外周血淋巴细胞中的微核频率被广泛用作人类群体中染色体损伤和基因组稳定性的生物标志物。我们研究了长期接触复杂农药混合物是否会导致细胞遗传学损伤增加。暴露组由50名农业飞行员组成，主要来自巴西中部和东南部地区，他们在没有个人防护设备的情况下吸入农用化学品超过10年；对照组由来自同一地区的17名男性组成，没有接触农药的迹象。与对照组相比，飞行员的微核频率高出三倍（P < 0.05），双核细胞频率高出2.5倍。然而，暴露组和对照组之间的细胞毒性改变，如破碎的细胞和核溶解，没有统计学上的显著差异。因此，本研究表明，农业中用于防治害虫的各种农用化学品对农业飞行员的口腔黏膜具有遗传毒性作用。
