Division of Hygiene and Medical Microbiology, Innsbruck Medical University, Schöpfstr. 41/2, 6020 Innsbruck, Austria.
J Med Microbiol. 2012 Apr;61(Pt 4):567-571. doi: 10.1099/jmm.0.038646-0. Epub 2011 Dec 22.
The rapid emergence and dissemination of carbapenemase-producing Enterobacter species and other members of the Enterobacteriaceae poses a considerable threat to the care of hospitalized patients and to public health. In this study, Enterobacter isolates demonstrating decreased susceptibility to carbapenems detected at the Division of Hygiene and Medical Microbiology, Innsbruck Medical University, between January 2006 and December 2010 were tested for bla(VIM-1), bla(NDM-1), bla(IMP), bla(KPC) and bla(OXA-48) using a multiplex PCR with published primers. PFGE was performed to determine the genetic relatedness. In total, 33 isolates (28 Enterobacter cloacae and 5 Enterobacter aerogenes) were collected during the study period. From 2006 to 2009, between two and seven isolates were found per year. In 2010, a significant increase of carbapenem-resistant strains was observed (n = 12). The bla(VIM-1) gene was detected in all 28 isolates of E. cloacae. Typing of E. cloacae by PFGE revealed three distinct clusters, the biggest of which contained 18 isolates. These findings demonstrate the emergence of VIM-1-producing Enterobacter in Tyrol, western Austria. The clonal relationship confirms the risk of spread of these organisms and their possible persistence over time.
产碳青霉烯酶肠杆菌科细菌和其他肠杆菌科成员的迅速出现和传播对住院患者的治疗和公共健康构成了相当大的威胁。在这项研究中，使用发表的引物通过多重 PCR 检测到 2006 年 1 月至 2010 年 12 月期间在因斯布鲁克医科大学卫生和医学微生物学系发现的对碳青霉烯类药物敏感性降低的肠杆菌属分离物中的 bla(VIM-1)、bla(NDM-1)、bla(IMP)、bla(KPC)和 bla(OXA-48)。进行 PFGE 以确定遗传相关性。在此期间共收集了 33 株分离株（28 株阴沟肠杆菌和 5 株产气肠杆菌）。2006 年至 2009 年，每年发现 2 至 7 株分离株。2010 年，观察到耐碳青霉烯菌株的数量显著增加（n = 12）。bla(VIM-1)基因在所有 28 株阴沟肠杆菌中均被检测到。PFGE 对阴沟肠杆菌的分型显示出三个不同的簇，其中最大的簇包含 18 株分离株。这些发现表明，产 VIM-1 的肠杆菌在奥地利西部的蒂罗尔州出现。PFGE 的克隆关系证实了这些生物体传播的风险及其可能随时间持续存在的可能性。
