Instituto de Física, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
PLoS One. 2011;6(12):e29244. doi: 10.1371/journal.pone.0029244. Epub 2011 Dec 16.
The mechanisms underlying pluripotency and differentiation in embryonic and reprogrammed stem cells are unclear. In this work, we characterized the pluripotent state towards neural differentiated state through analysis of trace elements distribution using the Synchrotron Radiation X-ray Fluorescence Spectroscopy. Naive and neural-stimulated embryoid bodies (EB) derived from embryonic and induced pluripotent stem (ES and iPS) cells were irradiated with a spatial resolution of 20 µm to make elemental maps and qualitative chemical analyses. Results show that these embryo-like aggregates exhibit self-organization at the atomic level. Metallic elements content rises and consistent elemental polarization pattern of P and S in both mouse and human pluripotent stem cells were observed, indicating that neural differentiation and elemental polarization are strongly correlated.
胚胎和重编程干细胞中多能性和分化的机制尚不清楚。在这项工作中，我们通过同步辐射 X 射线荧光光谱分析微量元素分布来描述多能性向神经分化状态。使用空间分辨率为 20µm 的同步辐射 X 射线荧光光谱术对来源于胚胎干细胞和诱导多能干细胞的原始态和神经刺激态胚状体（EB）进行照射，以制作元素图谱和定性化学分析。结果表明，这些类胚胎聚集物在原子水平上表现出自组织特性。金属元素含量增加，并且在人和鼠的多能干细胞中观察到 P 和 S 的一致元素极化模式，表明神经分化和元素极化密切相关。
