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人牙髓和根尖乳头干细胞:形态学与同步辐射分析

Stem cells from human dental pulp and apical papilla: Morphological and synchrotron radiation analysis.

作者信息

Rezende Karla-Mayra, Bönecker Marcelo, Côrrea Luciana, Perez Carlos-Alberto, Brito Giancarlo-Espósito-de Souza, Berti Gabriela-Oliveira, Pobocik Andrea-Mantesso

机构信息

Universidade de São Paulo - USP, School of Dentistry, Department of Pediatric Dentistry and Orthodontics, São Paulo, SP, Brazil.

Pathology Department, School of Dentistry, University of São Paulo, Brazil.

出版信息

J Clin Exp Dent. 2021 Dec 1;13(12):e1249-e1257. doi: 10.4317/jced.58819. eCollection 2021 Dec.

Abstract

BACKGROUND

Dental Mesenchymal stem cells has prompted great for cell-based therapeutics. But no one knows for sure what the true potential of these cells, since most of the studies were done in isolation, using as source, different donors or different cell processing conditions.

MATERIAL AND METHODS

An enriched population of cells positive for CD146, STRO-1, and CD90 was isolated of third molars teeth indicated for extraction of patient with of 16 years old. Analysis of cell kinetics, and subcellular tests were performed to assess the presence of minor and trace elements by using synchrotron radiation x-ray fluorescence microscopy.

RESULTS

In the cell kinetics assays, the enriched populations showed generally slower growth as compared to those that were non-enriched. In comparison between the pulp and papilla populations, the derived pulp grew more rapidly than that derived from the papilla. The CD90 + cells exhibited a smaller pulp area compared to other populations, but the papilla of these cells exhibited a larger area. The CD90 + cells exhibited higher amounts of P, S, Cl, K, and Ca, while the Cu and Zn exhibited more than CD146-. STRO1 - exhibited K and Cu. For both the pulp and the papilla, multipotent stem cells positive for all three markers were present.

CONCLUSIONS

Although they have been obtained from the same tooth and donor, as well as were grown, the populations derived from these two tissues have different growth morphology and kinetics. The biochemical differences show different metabolic patterns, reflecting in part the growth differences. Synchrotron radiation, dental stem cells, mesenchymal stem cells, chemical composition.

摘要

背景

牙间充质干细胞在基于细胞的治疗方面具有巨大潜力。但由于大多数研究是在分离状态下进行的,使用的是不同供体或不同细胞处理条件作为来源,所以没有人确切知道这些细胞的真正潜力。

材料与方法

从一名16岁患者拟拔除的第三磨牙中分离出一群CD146、STRO-1和CD90呈阳性的富集细胞。通过同步辐射X射线荧光显微镜进行细胞动力学分析和亚细胞测试,以评估微量元素的存在情况。

结果

在细胞动力学试验中,与未富集的细胞群体相比,富集群体的生长通常较慢。在牙髓和牙乳头群体之间的比较中,牙髓来源的细胞比牙乳头来源的细胞生长得更快。与其他群体相比,CD90+细胞的牙髓面积较小,但这些细胞的牙乳头面积较大。CD90+细胞中P、S、Cl、K和Ca的含量较高,而Cu和Zn的含量比CD146-细胞中的更多。STRO1-细胞中K和Cu的含量较高。对于牙髓和牙乳头,均存在对所有三种标志物呈阳性的多能干细胞。

结论

尽管这些细胞群体来自同一颗牙齿和同一个供体,并且是在相同条件下培养的,但来自这两种组织的细胞群体具有不同的生长形态和动力学。生化差异显示出不同的代谢模式,部分反映了生长差异。同步辐射、牙干细胞、间充质干细胞、化学成分。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0196/8715560/34363785cd81/jced-13-e1249-g001.jpg

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